%0 Journal Article
%T 沉默stathmin基因增强食管鳞癌细胞对紫杉醇敏感度的实验
%A Javed Akhtar
%A 王帅
%A 王洲
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.02.006
%X 摘要 目的 探讨沉默stathmin基因后，食管鳞癌细胞对紫杉醇敏感度的改变。方法 利用RNA干扰技术特异性沉默食管鳞癌KYSE150细胞的stathmin基因；实时定量PCR及蛋白质印迹法检测stathmin表达变化；CCK-8检测紫杉醇敏感度变化；流式细胞仪检测细胞周期变化；细胞分裂指数实验观察细胞有丝分裂改变。结果 特异性转染后，stathmin基因mRNA 和蛋白表达均受抑制。沉默stathmin基因后，KYSE150细胞对紫杉醇敏感度增强191.4倍；紫杉醇对特异性转染和未转染的KYSE150细胞半数抑制浓度分别为0.018 nM和3.445 nM。经紫杉醇（0.01 nM）处理72 h后，相比于未转染细胞，特异性转染的KYSE150细胞出现明显G2/M周期阻滞[（55.4±6.2%）vs.（19.1±2.7%）, P=0.000]， 有丝分裂指数减少[（5.6±0.8%）vs.（13.5±3.7%）, P=0.000]。结论 stathmin基因沉默通过细胞周期G2/M阻滞增强食管鳞癌细胞对紫杉醇的化疗敏感度
%K A Meta-analysis
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%K Clinical Investigation on Qingfei Mixture Combined with Chemotherapy on Middle and Advanced Non-small Cell Lung Cancer
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%K Bioinformatics Analysis of Genes Related to Multidrug Resistance in Ovarian Cancer
%U http://www.zlfzyj.com/CN/abstract/abstract8414.shtml