量子点分子探针双标记技术研究癌细胞克隆生长行为
DOI: 10.3971/j.issn.1000-8578.2015.02.001
Keywords: 细胞克隆形成,量子点,Ki67,Expressions and Significance of Notch2 Protein in Laryngeal Squamous Cell Carcinoma,Expression and Clinical Significance of usp22 and ki67 in Colorectal Carcinoma,Animal Model and Clinical Studies of Relationship between Ki67 Expression and 18F-FDG PET-CT Imaging of Nasopharyngeal Carcinoma before and after Radiotherapy,Overexpression and Clinicopathological Significance of NET-1,Ki67 and CD34 in Gastric Carcinoma,Expression of Smac and Ki67 in Thyroid Carcinoma and Its Cl inical Signif icance,Expression of Smac and Ki67 in Gastric Cancer and Its Clinical Significance,Antitumor Effect of Replication-competent Adenovirus Expressing Short Interference RNA Targeting Ki67 Gene on Lung Cancer,Expression and Clinical Significance of survivin 、VEGF and Ki67 in Colorectal Carcinoma,Effects of Peptide Nucleic Acids Targeting Ki67 on the Prol iferation and Apoptosis of Human Renal Carcinoma Cell Line,Relationship between Cyclooxygenase-2 and Ki67 Expression in Transitional Cell Carcinoma of Bladder and Its Significance,Expression and Clinical Significance of P-GP170, LRP and Ki-67 in Thyroid Carcinoma,Expression of FHIT, Ki67 and bax and Clinical Significance in Cervix Carcinoma,Effects of Mifepristone on Cell Proliferation of Human Ovarian Epithelial Carcinoma Cell Line HO_(8910) in Vitro,Significance and Expression of survivin Protein and Ki67 in Non-hodgkin′s Lymphoma,The significance of the expression of Ki67 antigen in nasopharyngeal carcinoma
Abstract:
摘要 目的 研究癌细胞的克隆生长行为，分析癌细胞的增殖特点。方法 以乳腺癌MCF-7细胞、结肠癌SW480细胞和胃癌SGC7901细胞为研究对象，进行细胞克隆的形态学特点研究。用量子点分子探针双色标记技术，以绿色量子点标记癌细胞胞质，黄、红色量子点标记癌细胞胞核Ki67，观察癌细胞克隆的形成速度，克隆内细胞形态、离散趋势、Ki67表达分布情况。结果 细胞克隆形成实验可见，乳腺癌MCF-7细胞生长6天形成克隆，结肠癌SW480细胞生长8天形成克隆，胃癌SGC7901细胞生长12天形成克隆。三种细胞均具有明显异型性特点；核质比大，易见病理性核分裂相；克隆周边细胞形成较多突触。部分克隆中，边缘细胞与克隆分离或呈分离趋势。量子点探针技术检测可见，大部分Ki67强阳性细胞分布于克隆周边或集中于一侧。结论 Ki67广泛表达于MCF-7、SW480和SGC7901三种癌细胞，且在癌细胞克隆周边表达更强或集中于一侧，癌细胞克隆呈现明显的不对称生长行为
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