%0 Journal Article
%T 量子点分子探针双标记技术研究癌细胞克隆生长行为
%A 刘少平
%A 方敏
%A 李雁
%A 耿霞飞
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.02.001
%X 摘要 目的 研究癌细胞的克隆生长行为，分析癌细胞的增殖特点。方法 以乳腺癌MCF-7细胞、结肠癌SW480细胞和胃癌SGC7901细胞为研究对象，进行细胞克隆的形态学特点研究。用量子点分子探针双色标记技术，以绿色量子点标记癌细胞胞质，黄、红色量子点标记癌细胞胞核Ki67，观察癌细胞克隆的形成速度，克隆内细胞形态、离散趋势、Ki67表达分布情况。结果 细胞克隆形成实验可见，乳腺癌MCF-7细胞生长6天形成克隆，结肠癌SW480细胞生长8天形成克隆，胃癌SGC7901细胞生长12天形成克隆。三种细胞均具有明显异型性特点；核质比大，易见病理性核分裂相；克隆周边细胞形成较多突触。部分克隆中，边缘细胞与克隆分离或呈分离趋势。量子点探针技术检测可见，大部分Ki67强阳性细胞分布于克隆周边或集中于一侧。结论 Ki67广泛表达于MCF-7、SW480和SGC7901三种癌细胞，且在癌细胞克隆周边表达更强或集中于一侧，癌细胞克隆呈现明显的不对称生长行为
%K 细胞克隆形成
%K 量子点
%K Ki67
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%K Expression and Clinical Significance of usp22 and ki67 in Colorectal Carcinoma
%K Animal Model and Clinical Studies of Relationship between Ki67 Expression and 18F-FDG PET-CT Imaging of Nasopharyngeal Carcinoma before and after Radiotherapy
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%K Expression of Smac and Ki67 in Thyroid Carcinoma and Its Cl inical Signif icance
%K Expression of Smac and Ki67 in Gastric Cancer and Its Clinical Significance
%K Antitumor Effect of Replication-competent Adenovirus Expressing Short Interference RNA Targeting Ki67 Gene on Lung Cancer
%K Expression and Clinical Significance of survivin 、VEGF and Ki67 in Colorectal Carcinoma
%K Effects of Peptide Nucleic Acids Targeting Ki67 on the Prol iferation and Apoptosis of Human Renal Carcinoma Cell Line
%K Relationship between Cyclooxygenase-2 and Ki67 Expression in Transitional Cell Carcinoma of Bladder and Its Significance
%K Expression and Clinical Significance of P-GP170
%K LRP and Ki-67 in Thyroid Carcinoma
%K Expression of FHIT
%K Ki67 and bax and Clinical Significance in Cervix Carcinoma
%K Effects of Mifepristone on Cell Proliferation of Human Ovarian Epithelial Carcinoma Cell Line HO_(8910) in Vitro
%K Significance and Expression of survivin Protein and Ki67 in Non-hodgkin′s Lymphoma
%K The significance of the expression of Ki67 antigen in nasopharyngeal carcinoma
%U http://www.zlfzyj.com/CN/abstract/abstract8409.shtml