小分子化合物PHA-767491对白血病K562细胞抑制增殖和诱导凋亡的作用
DOI: 10.3971/j.issn.1000-8578.2015.03.006
Keywords: Cdc7激酶,PHA-767491,K562,凋亡,Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells,Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88,siRNA-mediated Inhibition of MTH1 Gene Expression Promotes Apoptosis and Reduces Migration of Hepatocellular Carcinoma HepG2 Cells,Effect of SAHA on Proliferation and Apoptosis of Breast Cancer Cells MDA-MB-435 and Related Mechanism,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism,Progress of Relationships of Calprotectin with Tumours,Experimental Investigation on Apoptosis of Cervical Cancer Cells Promoted by NF-κB Decoy ODN,Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation,Effect of Terpinen-4-ol on Proliferation and Apoptosis of Human Multiple Myeloma Cells U266,Periplocin Extracted from Cortex Periplocae(CPP) Induced Apoptosis of Gastric Cancer Cells MGC-803 Through Lysosomal Pathway,Research Advances of ASPP in Regulating Apoptosis of Tumor Cells,Effect of AT Motif Binding Factor 1 on Proliferation and Invasion of Colorectal Carcinoma Cell Line LOVO,Reversion Impact of Peptide Extract from Scorpion Venom on Multidrug-resistance of Leukemia Stem Cell Line K562/A02 in vivo,Ionizing Radiation Overcomes Gefitinib Resistance of Non-small Cell Lung Cancer Cell Line H1975 in vitro
Abstract:
摘要 目的 探讨PHA-767491对白血病细胞K562的抑制能力及分子机制。方法 采用不同浓度的PHA-767491作用于K562细胞，利用MTT法在不同的时间点检测细胞存活率，RT-PCR及Western blot检测PHA-767491诱导的凋亡信号途径，hochest染色及流式细胞术检测K562的凋亡水平。结果 PHA-767491显著抑制K562细胞的增殖，进一步结果表明PHA-767491可以显著下调抗凋亡蛋白Xiap和Mcl-1蛋白的表达水平，诱导K562细胞通过Caspase途径进入凋亡。结论 PHA-767491可以通过诱导K562细胞凋亡而显著抑制其增殖，为今后的白血病治疗提供了一种新的备选药物
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