转录因子Snail对食管癌Eca-109细胞侵袭和迁移的影响
DOI: 10.3971/j.issn.1000-8578.2015.04.001
Keywords: 食管癌,Snail,上皮间质转化,侵袭,迁移,NDRG2 Inhibits Migration of Breast Cancer Cells Through Upregulating PTEN Expression,Clinical Analysis of Radiotherapy Combined with Cisplatin-based Chemotherapy for Locally Advanced Esophageal Squamous Cell Carcinoma,Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells,Mechanism of Ibuprofen Regulating Migration and Invasion of Liver Cancer QGY-7703 Cells Through PI3K/Akt/mTOR Signaling Pathway,Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells,Effect of Jinlong Capsule on Vasculogenic Mimicry and Mig-7 of Colon Cancer,siRNA-mediated Inhibition of MTH1 Gene Expression Promotes Apoptosis and Reduces Migration of Hepatocellular Carcinoma HepG2 Cells,Effect of PTTG1 Expression on Invasion and Migration of Colon Cancer Cell SW480 and Its Possible Mechanism,Effect of RORα Overexpression on Proliferation, Migration and Invasion of Human Gastric MGC803 Cells,Relationship Between NF-κB and Epithelial-to-mesenchymal Transition of Radiationinduced Human Esophageal Carcinoma Cells,Upper Gastrointestinal Carcinomas Incidence from 2003 to 2012 in Cixian, Hebei Province,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Department of Thoracic Surgery, Zhejiang Cancer Hospital, Hangzhou 310022, China,Effect of Ectopic Expression of Transmembrane Adaptor PAG1 on Motility of Cutaneous Squamous Cell Carcinoma A431 Cells in vitro,Effects on Cell Proliferation, Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway
Abstract:
摘要 目的 探讨Snail在诱导食管癌细胞侵袭和迁移中的作用及机制。方法 采用慢病毒转染构建食管癌细胞株Eca-109-Snail和Eca-109-vc，Real-time PCR及Western blot实验分别检测其mRNA及蛋白表达水平，Transwell及细胞划痕实验检测细胞侵袭和迁移能力。 结果 慢病毒转染细胞株构建成功。经Real-time PCR及Western blot实验鉴定慢病毒转染后的Eca-109-Snail细胞Snail表达量升高，细胞表现出成纤维细胞样外形，细胞之间彼此分离；Eca-109-Snail细胞E-cadherin表达下降，Vimentin和MMP-2表达上升，其侵袭和迁移能力较空载体对照组增强，差异均具有统计学意义（P<0.01）。 结论 Snail通过诱导食管癌Eca-109细胞发生上皮间质转化，进而提高其侵袭和迁移能力
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