%0 Journal Article
%T 转录因子Snail对食管癌Eca-109细胞侵袭和迁移的影响
%A 张琳丽
%A 李文雯
%A 胡国清
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.04.001
%X 摘要 目的 探讨Snail在诱导食管癌细胞侵袭和迁移中的作用及机制。方法 采用慢病毒转染构建食管癌细胞株Eca-109-Snail和Eca-109-vc，Real-time PCR及Western blot实验分别检测其mRNA及蛋白表达水平，Transwell及细胞划痕实验检测细胞侵袭和迁移能力。 结果 慢病毒转染细胞株构建成功。经Real-time PCR及Western blot实验鉴定慢病毒转染后的Eca-109-Snail细胞Snail表达量升高，细胞表现出成纤维细胞样外形，细胞之间彼此分离；Eca-109-Snail细胞E-cadherin表达下降，Vimentin和MMP-2表达上升，其侵袭和迁移能力较空载体对照组增强，差异均具有统计学意义（P<0.01）。 结论 Snail通过诱导食管癌Eca-109细胞发生上皮间质转化，进而提高其侵袭和迁移能力
%K 食管癌
%K Snail
%K 上皮间质转化
%K 侵袭
%K 迁移
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%K Hangzhou 310022
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%U http://www.zlfzyj.com/CN/abstract/abstract8457.shtml