曲古抑菌素A增加人肝癌Bel7402细胞对TRAIL敏感度的实验
DOI: 10.3971/j.issn.1000-8578.2015.06.002
Keywords: 肝细胞癌细胞,曲古抑菌素A,肿瘤坏死因子相关凋亡诱导配体,细胞凋亡,Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism,Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation,Effect of AT Motif Binding Factor 1 on Proliferation and Invasion of Colorectal Carcinoma Cell Line LOVO,Ionizing Radiation Overcomes Gefitinib Resistance of Non-small Cell Lung Cancer Cell Line H1975 in vitro,Effects of Gefitinib Combined with Bruceolic Oil Emulsion on Human Lung Adenocarcinoma Cell Lines and Its Mechanism,Tumor Suppressor Roles of miR-100 in Esophageal Squamous Cell Carcinoma Cell Line Ec-109,Effect of microRNA-7 Overexpression on Apoptosis of Human Lung Cancer Cell Line 95D,Mechanism of TG2 Regulating Hypoxia-induced Apoptosis in Osteosarcoma Cell Line MG-63 by Mitochondrial Pathway,Sequence-dependent Effects and Mechanism of Pemetrexed and Gefitinib on Human Lung Adenocarcinoma Cells,Embelin Synergistically Enhances TRAIL-induced Apoptotic Death in Breast Cancer Cell Line MDA-MB-231,Effects of Bufalin on Proliferation and Apoptosis of Melanoma Cells B16,Influence of Microenvironment pH Value on Growth of Tumor Cells,Effect of Ammonium Molybdate on Cell Proliferation and Cycle of Hepatoma and Colon Cancer
Abstract:
摘要 目的 探讨曲古抑菌素 A（Trichostatin A, TSA）联合肿瘤坏死因子（tumor necrosis factor, TNF）相关凋亡诱导配体（TNF related apoptosis inducing ligand, TRAIL）对肝癌细胞Bel7402增殖凋亡的影响及其机制。方法 采用四甲基偶氮唑盐（MTT）染色法分别检测TSA、TRAIL及低浓度TSA联合TRAIL处理Bel7402细胞的生长抑制率；4,6-二脒基-2-苯基吲哚二盐酸盐（DAPI）染色法对药物联合处理后的细胞进行凋亡形态学观察；免疫细胞化学和Western blot技术观察药物联合作用后p65蛋白在细胞中表达和定位的变化。结果 不同浓度TSA作用6、12和24 h对人肝癌Bel7402细胞的增殖没有明显抑制作用，而作用48 h后的细胞增殖抑制率明显升高，和对照组比较差异有统计学意义（P<0.05）；不同浓度TRAIL处理Bel7402细胞存活率没有明显改变；低浓度TSA（20 ng/ml）预处理能够增加Bel7402细胞对TRAIL治疗的敏感度，TSA预处理联合TRAIL（100ng/ml)作用细胞24 h后，细胞生存率为（57.1±5.4）%，和单独药物处理组及对照组比较差异有统计学意义（P<0.05）；DAPI染色显示TSA和TRAIL联合作用后Bel7402细胞有核凋亡出现。荧光显微镜观察证明单独应用TSA（200 ng/ml）或TRAIL（100 ng/ml）处理的细胞p65蛋白有部分核内移位，而两种药物联合应用导致p65蛋白表达量降低，并且发生明显的核内转移和集聚。结论 低浓度TSA能够增加肝癌Bel7402细胞对TRAIL的敏感度；其机制可能是两种药物联合应用降低p65的表达和活性，从而诱导Bel7402细胞凋亡
Full-Text
