%0 Journal Article
%T 曲古抑菌素A增加人肝癌Bel7402细胞对TRAIL敏感度的实验
%A 夏华
%A 朱明月
%A 李伟
%A 李孟森
%A 符史干
%A 董栩
%A 谢协驹
%A 郭峻莉
%A 陈栘
%A 鲁琰
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.06.002
%X 摘要 目的 探讨曲古抑菌素 A（Trichostatin A, TSA）联合肿瘤坏死因子（tumor necrosis factor, TNF）相关凋亡诱导配体（TNF related apoptosis inducing ligand, TRAIL）对肝癌细胞Bel7402增殖凋亡的影响及其机制。方法 采用四甲基偶氮唑盐（MTT）染色法分别检测TSA、TRAIL及低浓度TSA联合TRAIL处理Bel7402细胞的生长抑制率；4,6-二脒基-2-苯基吲哚二盐酸盐（DAPI）染色法对药物联合处理后的细胞进行凋亡形态学观察；免疫细胞化学和Western blot技术观察药物联合作用后p65蛋白在细胞中表达和定位的变化。结果 不同浓度TSA作用6、12和24 h对人肝癌Bel7402细胞的增殖没有明显抑制作用，而作用48 h后的细胞增殖抑制率明显升高，和对照组比较差异有统计学意义（P<0.05）；不同浓度TRAIL处理Bel7402细胞存活率没有明显改变；低浓度TSA（20 ng/ml）预处理能够增加Bel7402细胞对TRAIL治疗的敏感度，TSA预处理联合TRAIL（100ng/ml)作用细胞24 h后，细胞生存率为（57.1±5.4）%，和单独药物处理组及对照组比较差异有统计学意义（P<0.05）；DAPI染色显示TSA和TRAIL联合作用后Bel7402细胞有核凋亡出现。荧光显微镜观察证明单独应用TSA（200 ng/ml）或TRAIL（100 ng/ml）处理的细胞p65蛋白有部分核内移位，而两种药物联合应用导致p65蛋白表达量降低，并且发生明显的核内转移和集聚。结论 低浓度TSA能够增加肝癌Bel7402细胞对TRAIL的敏感度；其机制可能是两种药物联合应用降低p65的表达和活性，从而诱导Bel7402细胞凋亡
%K 肝细胞癌细胞
%K 曲古抑菌素A
%K 肿瘤坏死因子相关凋亡诱导配体
%K 细胞凋亡
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%U http://www.zlfzyj.com/CN/abstract/abstract8507.shtml