南方红豆杉水提物逆转非小细胞肺癌干细胞耐药作用的实验
DOI: 10.3971/j.issn.1000-8578.2016.11.003
Keywords: A Meta-analysis,Effect of Rap2a on Invasion and Metastasis of Lung Cancer Cells and Its Related Mechanism,Immune Killing Effect of Silencing TIPE2 Expression on T Lymphocytes Against Lung Adenocarcinoma Cells LA795,NSCLC的疫苗治疗研究进展,Experiment of Different Administration Sequences of Apatinib and Paclitaxel on Lung Cancer,Expressions of MACC1 and c-Met Proteins in Lung Adenocarcinoma and Their Correlation with Postoperative Recurrence,Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation,Clinical Investigation on Qingfei Mixture Combined with Chemotherapy on Middle and Advanced Non-small Cell Lung Cancer,Effect of Vascular Endothelial Cadherin on Invasion and Metastasis of Non-small Cell Lung Cancer Cells and Related Mechanism,Advances of FGF/FGFR Signaling Pathway in Targeted Therapy for Squamous Cell Lung Cancer
Abstract:
摘要 目的 研究南方红豆杉水提物逆转非小细胞肺癌干细胞耐药作用的机制。方法 选取人非小细胞肺癌H460细胞为实验细胞，以侧群细胞及细胞表面标志物鉴定法筛选出其中的干细胞；CCK8（cell counting kit-8）法检测红豆杉水提物对H460干细胞的毒性，并检测红豆杉水提物逆转H460干细胞对顺铂的耐药性；流式细胞术（flow cytometry, FCM）检测红豆杉水提物逆转H460干细胞耐药的作用机制。结果 红豆杉水提物对H460干细胞有细胞毒性，测得H460干细胞的IC50为1 449.5 μg/ml；用红豆杉水提物预处理后的H460干细胞进行实验发现，顺铂的抗肿瘤效应明显增强，其作用机制为抑制细胞膜上的P-gp蛋白来降低有效药物排出量。结论 南方红豆杉水提物能够较强的逆转非小细胞肺癌干细胞的耐药性
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