%0 Journal Article
%T 南方红豆杉水提物逆转非小细胞肺癌干细胞耐药作用的实验
%A 余灿炳
%A 刘剑
%A 姜贻乾
%A 安素红
%A 徐佳灵
%A 徐小平
%A 苏芳
%A 莫永祥
%A 郭庆敏
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.11.003
%X 摘要 目的 研究南方红豆杉水提物逆转非小细胞肺癌干细胞耐药作用的机制。方法 选取人非小细胞肺癌H460细胞为实验细胞，以侧群细胞及细胞表面标志物鉴定法筛选出其中的干细胞；CCK8（cell counting kit-8）法检测红豆杉水提物对H460干细胞的毒性，并检测红豆杉水提物逆转H460干细胞对顺铂的耐药性；流式细胞术（flow cytometry, FCM）检测红豆杉水提物逆转H460干细胞耐药的作用机制。结果 红豆杉水提物对H460干细胞有细胞毒性，测得H460干细胞的IC50为1 449.5 μg/ml；用红豆杉水提物预处理后的H460干细胞进行实验发现，顺铂的抗肿瘤效应明显增强，其作用机制为抑制细胞膜上的P-gp蛋白来降低有效药物排出量。结论 南方红豆杉水提物能够较强的逆转非小细胞肺癌干细胞的耐药性
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%U http://www.zlfzyj.com/CN/abstract/abstract8865.shtml