重离子辐照对人舌鳞癌CAL27细胞的生物学效应及其分子机制
DOI: 10.3971/j.issn.1000-8578.2018.17.1673
Keywords: 重离子,舌鳞状细胞癌,P53,P65,VEGF,Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism,Research Progress of Combination Therapy of Apatinib on Tumor,Expression of TOPⅡα, TP53 and HER2 in Gastric Adenocarcinoma and Their Clinical Significance,Expression of IRSp53 in Epithelial Ovarian Carcinoma and Its Prognostic Significance,Comment on Investment Tide of Proton and Heavy Ion Irradiation,Effect of SAHA on Proliferation and Apoptosis of Breast Cancer Cells MDA-MB-435 and Related Mechanism,Correlation Between Expression of SIX1, TGF-β and VEGF-C in Primarily Cultured Human Laryngeal Squamous Cell Carcinoma Cells,Effect of Long Non-coding RNA LOC100294362 on Proliferation and Invasion of Breast Cancer Cells,Research Advances of ASPP in Regulating Apoptosis of Tumor Cells,Valuation of SUVmax, Ki-67, p53 and EGFR in Predicting Effect of Neoadjuvant Chemotherapy on Triple-negative Breast Cancer Patients,Effects of PPP2R5C Downregulation on Expression Profile of GSK-3β, MDM2, pTEN and TP53 Signal Pathway Regulating Genes in Jurkat Cells,Role of p53β Isoform in Inhibitory Effect of rmhTNF Combined with Cisplatin on Human Gastric Cancer Cell Growth,IDO Expression in Esophageal Squamous Cell Carcinoma and Its Relationship with Tumor Angiogenesis,Effect of CRM1 Inhibitor KPT-185 on Growth of Human Prostate Cancer Cells,Effect of Oxaliplatin on CD44V6, VEGF, survivin, Caspase-3 and Caspase-7 in Dimethylhydrazine-induced Colon Cancer Rats
Abstract:
摘要 目的 研究重离子12C6+离子辐照对人舌鳞癌CAL27细胞的生物学效应以及分子机制。方法 应用不同剂量重离子束（12C6+）对体外培养的人舌癌CAL27细胞进行辐照，采用MTT、Transwell、流式细胞术、划痕实验、克隆实验观察重离子束（12C6+）对CAL27细胞迁移和侵袭、凋亡和周期的影响，Western blot法观察重离子束（12C6+）对CAL27细胞增殖的影响。结果 （1）重离子辐照对CAL27细胞的增殖抑制作用与剂量-时间成正相关；（2）和空白对照组比较，在相同时间点，不同剂量的重离子束（12C6+）对细胞中P53、P65和VEGF的表达影响明显，随着重离子束（12C6+）辐照剂量增大，细胞中P53、P65和VEGF的表达明显降低（P<0.05）。结论 重离子辐照能抑制CAL27细胞的增殖，且具有剂量和时间效应
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