CLU基因干扰质粒的构建及其对鼻咽癌细胞生物学功能的影响
DOI: 10.3971/j.issn.1000-8578.2016.06.002
Keywords: 鼻咽癌,丛生蛋白,肿瘤转移,Comparison of Long-term Efficacy of IMRT plus Concurrent Chemotherapy Versus Neoadjuvant Chemotherapy Followed by IMRT plus Concurrent Chemotherapy for Locoregionally Advanced Nasopharyngeal Carcinoma,Correlation Between Skull Base Invasion and Bone Metastases in Locally Advanced Nasopharyngeal Carcinoma Patients,Research Progress of Neoadjuvant Chemotherapy for Locally Advanced Nasopharyngeal Carcinoma,Effect of 5-Azacytidine on hTERT mRNA Expression and Telomerase Activity in Human Nasopharyngeal Carcinoma Cells,Expression of Annexin A2 in Nasopharyngeal Carcinoma Tissues and Its Clinical Significance,Trends on Morbidity of Nasopharyngeal Carcinoma Among Residents in Pudong New Area of Shanghai, 2002-2013,New Progress on Tumor-associated Macrophages in Tumor Microenvironment,Effects of High Mobility Group Box-1 Protein on Migration and Invasion Ability of Nasopharyngeal Carcinoma Cell Line C666-1 in vitro,Role of Cancer-associated Fibroblasts in Occurrence and Development of Colorectal Cancer,Correlation of DNA Repair Gene XRCC1 Single Nucleotide Polymorphism with Sensitivity of Nasopharyngeal Carcinoma Patients to Platinum Chemotherapy,Progress of Correlation of Cell Membrane Phospholipids with Invasion, Metastasis and Targeted Therapeutics of Tumor,Expression of Specific Long Non-coding RNA in Nasopharyngeal Carcinoma and Related Significance,Cisplatin-induced Senescence and Senescence-related Gene Expression Profiles in#br# Human Nasopharyngeal Carcinoma Cells CNE2,Bergapten Induces Apoptosis of Nasopharyngeal Carcinoma Cells,VEGF Gene Promoter Region-460 T/C Single Nucleotide Polymorphisms and#br# Nasopharyngeal Carcinoma Susceptibility in Guangxi Population
Abstract:
摘要 目的 构建CLU基因干扰重组质粒及建立CLU基因干扰转染鼻咽癌细胞及动物模型，检测CLU对鼻咽癌细胞生物学功能的影响。方法 首先以real-time PCR和Western blot法验证CLU在鼻咽癌细胞系5-8F，6-10B中的表达，以高表达CLU的5-8F细胞作为干扰转染的目标细胞；设计及构建shRNA表达载体pSR-GFP/Neo-CLU-shRNA，将其转染至5-8F鼻咽癌细胞系中，通过Transwell小室迁移及侵袭实验来检测对其迁移和侵袭功能的影响。结果 与6-10B细胞比较，CLU基因在5-8F中的表达显著上调（P<0.05）；干扰鼻咽癌细胞5-8F中的CLU可使细胞迁移和侵袭功能明显减弱（P<0.05），使其体外成瘤能力显著降低（P<0.05）。结论 CLU基因在鼻咽癌细胞系5-8F中明显高表达；pSR-GFP/Neo-CLUshRNA 可成功干扰5-8F中CLU的表达；CLU可抑制5-8F细胞的迁移、侵袭及体内成瘤能力
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