%0 Journal Article
%T CLU基因干扰质粒的构建及其对鼻咽癌细胞生物学功能的影响
%A 卢金平
%A 李跃进
%A 王巍巍
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.06.002
%X 摘要 目的 构建CLU基因干扰重组质粒及建立CLU基因干扰转染鼻咽癌细胞及动物模型，检测CLU对鼻咽癌细胞生物学功能的影响。方法 首先以real-time PCR和Western blot法验证CLU在鼻咽癌细胞系5-8F，6-10B中的表达，以高表达CLU的5-8F细胞作为干扰转染的目标细胞；设计及构建shRNA表达载体pSR-GFP/Neo-CLU-shRNA，将其转染至5-8F鼻咽癌细胞系中，通过Transwell小室迁移及侵袭实验来检测对其迁移和侵袭功能的影响。结果 与6-10B细胞比较，CLU基因在5-8F中的表达显著上调（P<0.05）；干扰鼻咽癌细胞5-8F中的CLU可使细胞迁移和侵袭功能明显减弱（P<0.05），使其体外成瘤能力显著降低（P<0.05）。结论 CLU基因在鼻咽癌细胞系5-8F中明显高表达；pSR-GFP/Neo-CLUshRNA 可成功干扰5-8F中CLU的表达；CLU可抑制5-8F细胞的迁移、侵袭及体内成瘤能力
%K 鼻咽癌
%K 丛生蛋白
%K 肿瘤转移
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%U http://www.zlfzyj.com/CN/abstract/abstract8764.shtml