AT模体结合因子1对结直肠癌LOVO细胞增殖侵袭的影响
DOI: 10.3971/j.issn.1000-8578.2016.04.006
Keywords: AT模体结合因子1,抑癌基因,增殖,侵袭,细胞凋亡,Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells,Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88,Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells,Mechanism of Ibuprofen Regulating Migration and Invasion of Liver Cancer QGY-7703 Cells Through PI3K/Akt/mTOR Signaling Pathway,Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells,Effect of PTTG1 Expression on Invasion and Migration of Colon Cancer Cell SW480 and Its Possible Mechanism,Effect of RORα Overexpression on Proliferation, Migration and Invasion of Human Gastric MGC803 Cells,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Effect of Ectopic Expression of Transmembrane Adaptor PAG1 on Motility of Cutaneous Squamous Cell Carcinoma A431 Cells in vitro,Effects on Cell Proliferation, Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway,Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism,Effect of Rap2a on Invasion and Metastasis of Lung Cancer Cells and Its Related Mechanism,Progress of TBX3 in Development of Malignant Tumors,Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation,Effect of Vascular Endothelial Cadherin on Invasion and Metastasis of Non-small Cell Lung Cancer Cells and Related Mechanism
Abstract:
摘要 目的 构建ATBF1高表达的LOVO细胞，并观察其增殖性、凋亡率、侵袭性的变化。方法 将pEGFP-ATBF1转染入LOVO细胞后，在荧光显微镜下观察其转染情况，通过RT-PCR、Western blot检测并研究转染后ATBF1、Notch3的表达情况。利用CCK-8（Cell Counting Kit-8）和小室迁移实验分别研究LOVO细胞的增殖状态与侵袭能力，通过流式细胞术探索ATBF1对LOVO凋亡的影响。结果 与空白组（未处理组）、对照组（转染pEGFP质粒）对比，实验组（转染pEGFP-ATBF1质粒）LOVO细胞的增殖性、侵袭性明显受到抑制（P<0.001），流式细胞术提示实验组LOVO细胞的凋亡率为15.9%，空白组和对照组的凋亡率仅为5.2%和4.6%，而Western blot分析结果表明实验组癌基因Notch3表达量较其他两组有所下降（P<0.05）。结论 抑癌基因ATBF1可抑制LOVO的增殖性及侵袭性，并促进其凋亡，同时可下调Notch3的表达，这意味着Notch3有可能是ATBF1的一个下游基因
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