%0 Journal Article
%T AT模体结合因子1对结直肠癌LOVO细胞增殖侵袭的影响
%A 杨少华
%A 林志群
%A 莫林海
%A 赵大川
%A 赵海军
%A 陈飞
%A 黄宗海
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.04.006
%X 摘要 目的 构建ATBF1高表达的LOVO细胞，并观察其增殖性、凋亡率、侵袭性的变化。方法 将pEGFP-ATBF1转染入LOVO细胞后，在荧光显微镜下观察其转染情况，通过RT-PCR、Western blot检测并研究转染后ATBF1、Notch3的表达情况。利用CCK-8（Cell Counting Kit-8）和小室迁移实验分别研究LOVO细胞的增殖状态与侵袭能力，通过流式细胞术探索ATBF1对LOVO凋亡的影响。结果 与空白组（未处理组）、对照组（转染pEGFP质粒）对比，实验组（转染pEGFP-ATBF1质粒）LOVO细胞的增殖性、侵袭性明显受到抑制（P<0.001），流式细胞术提示实验组LOVO细胞的凋亡率为15.9%，空白组和对照组的凋亡率仅为5.2%和4.6%，而Western blot分析结果表明实验组癌基因Notch3表达量较其他两组有所下降（P<0.05）。结论 抑癌基因ATBF1可抑制LOVO的增殖性及侵袭性，并促进其凋亡，同时可下调Notch3的表达，这意味着Notch3有可能是ATBF1的一个下游基因
%K AT模体结合因子1
%K 抑癌基因
%K 增殖
%K 侵袭
%K 细胞凋亡
%K Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells
%K Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88
%K Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells
%K Mechanism of Ibuprofen Regulating Migration and Invasion of Liver Cancer QGY-7703 Cells Through PI3K/Akt/mTOR Signaling Pathway
%K Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells
%K Effect of PTTG1 Expression on Invasion and Migration of Colon Cancer Cell SW480 and Its Possible Mechanism
%K Effect of RORα Overexpression on Proliferation
%K Migration and Invasion of Human Gastric MGC803 Cells
%K Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients
%K Effect of Ectopic Expression of Transmembrane Adaptor PAG1 on Motility of Cutaneous Squamous Cell Carcinoma A431 Cells in vitro
%K Effects on Cell Proliferation
%K Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway
%K Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism
%K Effect of Rap2a on Invasion and Metastasis of Lung Cancer Cells and Its Related Mechanism
%K Progress of TBX3 in Development of Malignant Tumors
%K Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation
%K Effect of Vascular Endothelial Cadherin on Invasion and Metastasis of Non-small Cell Lung Cancer Cells and Related Mechanism
%U http://www.zlfzyj.com/CN/abstract/abstract8727.shtml