正电子核素68Ga标记PSMA靶向分子探针在神经胶质瘤模型中的应用
DOI: 10.3971/j.issn.1000-8578.2018.17.0405
Keywords: 前列腺特异性膜抗原,新生血管,正电子发射断层扫描,神经胶质瘤,镓-68,Effects for Histone Deacetylase Inhibitors on Proliferation and MKK7 Expression in Glioma Cells,Preparation and Evaluation of Neovessel-targeted Paramagnetic PEGylated Liposome,Effect of Bcl-2 Downregulation on Proliferation, Apoptosis and Invasion of Glioma Cell Lines,Aberrant Expression of NKG2D Ligand and High Expression HLA-Ⅰ Molecules May Contribute to Immune Escape from Natural Killer Cell-mediated Immune Surveillance of Malignant Gliomas,Cloning of Human tumstatin Gene and Expression of Its Recombinant Protein,Abnormality of Conf iguration of Microvessel in Hepatocellular Carcinoma,Relation of Interleukin-8 Expression with Angiogenesis in Human Oral Souamous Cell Carcinoma,Inhibition of Proliferation and Induction of Apoptosis by Freeze Combined with rhTNF-α in Rat C6 Brain Glioma Cell,Relationship between bFGF expression and cellular proliferation in human glioma,Antitumor effect research of BRM,Relation between immune response and bystander effect when using tk gene to treat therapy glioma,The effects of COX-2 expression on vascular endothelial growth factor and angiogenesis in gastric carcinoma,Inhibitory Effect of Tumor Induced Angiogenesis by Ginsenoside Rg3
Abstract:
摘要 目的 探讨68Ga-PSMA-617在神经胶质瘤U87MG荷瘤鼠的显像情况。方法 将靶向前列腺特异性膜抗原（prostate specific membrane antigen, PSMA）的新型探针DKFZ-PSMA-617进行68Ga核素标记，利用Radio-TLC对68Ga-PSMA-617的放射化学纯度进行快速质控，然后进行细胞水平实验，尾静脉注射5和40 min后对U87MG荷瘤鼠进行micro-PET显像，同时对PSMA阻滞剂ZJ-43（25 mg/kg）共注射组进行注射后40 min的图像采集。结果 Radio-TLC对68Ga-PSMA-617的标记率及放化纯测定可在10min内完成，放化纯高达（99.0±1.9）%，细胞水平实验显示68Ga-PSMA-617分子探针的特异性，同时也显示U87MG细胞表面仅有较少量PSMA表达，而U87MG荷瘤鼠的micro-PET显像可见随时间延长肿瘤对PSMA靶向分子探针的放射性摄取的逐渐增高，靶与非靶比值从1.85±0.02（5 min）增长至3.62±0.175（40 min），且肿瘤对该探针的摄取可被PSMA阻滞剂ZJ-43所抑制。结论 68Ga-PSMA-617不仅可应用于前列腺癌的诊断，也有望应用于新生血管丰富的神经胶质瘤的诊断
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