%0 Journal Article
%T 正电子核素68Ga标记PSMA靶向分子探针在神经胶质瘤模型中的应用
%A 刘特立
%A 刘菲
%A 徐晓霞
%A 朱华
%A 杨志
%A 解清华
%A 郭晓轶
%A 韩雪迪
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.0405
%X 摘要 目的 探讨68Ga-PSMA-617在神经胶质瘤U87MG荷瘤鼠的显像情况。方法 将靶向前列腺特异性膜抗原（prostate specific membrane antigen, PSMA）的新型探针DKFZ-PSMA-617进行68Ga核素标记，利用Radio-TLC对68Ga-PSMA-617的放射化学纯度进行快速质控，然后进行细胞水平实验，尾静脉注射5和40 min后对U87MG荷瘤鼠进行micro-PET显像，同时对PSMA阻滞剂ZJ-43（25 mg/kg）共注射组进行注射后40 min的图像采集。结果 Radio-TLC对68Ga-PSMA-617的标记率及放化纯测定可在10min内完成，放化纯高达（99.0±1.9）%，细胞水平实验显示68Ga-PSMA-617分子探针的特异性，同时也显示U87MG细胞表面仅有较少量PSMA表达，而U87MG荷瘤鼠的micro-PET显像可见随时间延长肿瘤对PSMA靶向分子探针的放射性摄取的逐渐增高，靶与非靶比值从1.85±0.02（5 min）增长至3.62±0.175（40 min），且肿瘤对该探针的摄取可被PSMA阻滞剂ZJ-43所抑制。结论 68Ga-PSMA-617不仅可应用于前列腺癌的诊断，也有望应用于新生血管丰富的神经胶质瘤的诊断
%K 前列腺特异性膜抗原
%K 新生血管
%K 正电子发射断层扫描
%K 神经胶质瘤
%K 镓-68
%K Effects for Histone Deacetylase Inhibitors on Proliferation and MKK7 Expression in Glioma Cells
%K Preparation and Evaluation of Neovessel-targeted Paramagnetic PEGylated Liposome
%K Effect of Bcl-2 Downregulation on Proliferation
%K Apoptosis and Invasion of Glioma Cell Lines
%K Aberrant Expression of NKG2D Ligand and High Expression HLA-Ⅰ Molecules May Contribute to Immune Escape from Natural Killer Cell-mediated Immune Surveillance of Malignant Gliomas
%K Cloning of Human tumstatin Gene and Expression of Its Recombinant Protein
%K Abnormality of Conf iguration of Microvessel in Hepatocellular Carcinoma
%K Relation of Interleukin-8 Expression with Angiogenesis in Human Oral Souamous Cell Carcinoma
%K Inhibition of Proliferation and Induction of Apoptosis by Freeze Combined with rhTNF-α in Rat C6 Brain Glioma Cell
%K Relationship between bFGF expression and cellular proliferation in human glioma
%K Antitumor effect research of BRM
%K Relation between immune response and bystander effect when using tk gene to treat therapy glioma
%K The effects of COX-2 expression on vascular endothelial growth factor and angiogenesis in gastric carcinoma
%K Inhibitory Effect of Tumor Induced Angiogenesis by Ginsenoside Rg3
%U http://www.zlfzyj.com/CN/abstract/abstract9186.shtml