人乳腺癌相关成纤维原代细胞培养的改良方法
DOI: 10.3971/j.issn.1000-8578.2018.18.0428
Keywords: 28 Cases Report,Advances of FGF/FGFR Signaling Pathway in Targeted Therapy for Squamous Cell Lung Cancer,MDM2 siRNA Inhibits Angiogenesis by Reducing Vascular Endothelial Growth Factor Production in Breast Cancer Cells,Role of Akt-mediated Signaling Pathways in Tumor Lymphangiogenesis,Complementarity Determining Region 3 Repertoire of Clonal T Cell Receptor α Chain in Patients with Micrometastasis from Breast Cancer,Difference of Self-renewal and Tumorigenicity among Different Breast Cancer Stem Cells Subpopulations,Role of Cancer-associated Fibroblasts in Occurrence and Development of Colorectal Cancer,Relationship of ALDH1+/CD133+ Stem Cell-like Cells with Angiogenesis in Breast Invasive Ductal Carcinoma Tissues,Effects of MNNG on Proliferation, Cycle and Apoptosis of Kazakh Normal Esophageal Epithelial Cells,Construction and Identification of Recombinant Adenovirus Vector of Small Interfering RNA Targeting Basic Fibroblast Growth Factor,Association between Urinary Molybdenum and Risk of Breast Cancer
Abstract:
摘要 目的 通过对常见的人乳腺癌相关成纤维细胞（BCAFs）原代培养方法进行改良，建立一种快速高效的BCAFs原代培养方法。方法 取确诊为浸润性乳腺癌患者的癌组织标本，每个标本随机分成大小、质量相同的改良组和传统组，改良组用改良酶消化法（Ⅱ型胶原酶+透明质酸酶消化10 min）消化，传统组用传统酶消化法（Ⅰ型胶原酶消化8 h）消化。通过细胞计数、MTT和免疫荧光法比较两组消化方法获得的细胞总数、培养48 h后贴壁细胞的活性及BCAFs的纯度并在倒置显微镜下观察BCAFs原代细胞特征。结果 改良酶消化法获得的细胞量、48 h贴壁细胞的活性和细胞纯度均高于传统酶消化法（P<0.001）。获得的BCAFs原代细胞呈纺锤体形或长梭形，胞质内颗粒较多，细胞排列不规则。结论 本研究成功建立了一种高效快速的BCAFs原代培养的方法，为后期探究其在乳腺癌中的功能研究建立基础
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