%0 Journal Article
%T 人乳腺癌相关成纤维原代细胞培养的改良方法
%A 李智勇
%A 白雪
%A 蔡慧云
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.18.0428
%X 摘要 目的 通过对常见的人乳腺癌相关成纤维细胞（BCAFs）原代培养方法进行改良，建立一种快速高效的BCAFs原代培养方法。方法 取确诊为浸润性乳腺癌患者的癌组织标本，每个标本随机分成大小、质量相同的改良组和传统组，改良组用改良酶消化法（Ⅱ型胶原酶+透明质酸酶消化10 min）消化，传统组用传统酶消化法（Ⅰ型胶原酶消化8 h）消化。通过细胞计数、MTT和免疫荧光法比较两组消化方法获得的细胞总数、培养48 h后贴壁细胞的活性及BCAFs的纯度并在倒置显微镜下观察BCAFs原代细胞特征。结果 改良酶消化法获得的细胞量、48 h贴壁细胞的活性和细胞纯度均高于传统酶消化法（P<0.001）。获得的BCAFs原代细胞呈纺锤体形或长梭形，胞质内颗粒较多，细胞排列不规则。结论 本研究成功建立了一种高效快速的BCAFs原代培养的方法，为后期探究其在乳腺癌中的功能研究建立基础
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%U http://www.zlfzyj.com/CN/abstract/abstract9242.shtml