Gab2通过调节EMT促进胃癌的侵袭转移
DOI: 10.3971/j.issn.1000-8578.2018.17.0881
Keywords: 胃癌,SGC7901,Gab2,上皮间质转化,MMP-9,侵袭,miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17,Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Mechanism of miRNA-451 Regulating Resistance of Gastric Cancer Cells to 5-Fu Through MRP,Effect of Da Vinci Robot Radical Gastrectomy on Gastrointestinal Motility and Gastrointestinal Hormones of Gastric Cancer Patients,Effect of Down-regulation of HOXB7 Expression on Proliferation, Migration and Invasion Abilities of Hepatoma Cells,Curcumin Reverses Ionizing Radiation-induced Epithelial Mesenchymal Transformation of Non-small Cell Lung Cancer A549 Cells,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性,Related Factors and Risk Model Prediction of Pneumonia After ESD in Patients with Early Gastric Cancer,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer,Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein
Abstract:
摘要 目的 探讨Gab2在胃癌组织中的表达情况及其对胃癌细胞侵袭转移能力的影响与机制。方法 采用免疫组织化学SP法检测胃癌组织中Gab2、E-cadherin、N-cadherin及MMP-9的表达并分析其与淋巴结转移的关系。应用小RNA干扰技术，转染SGC7901细胞株，RT-PCR法检测瞬时转染后Gab2基因表达情况，体外侵袭实验检测细胞转染后侵袭能力的变化，Western blot法检测各蛋白的表达。结果 胃癌组织中Gab2的表达水平与淋巴结转移显著相关（P=0.002），与E-cadherin的表达负相关（r=-0.693, P=0.000），而与N-cadherin和MMP-9的表达水平正相关（r=0.407, P=0.021; r=0.335, P=0.017）。小RNA干扰技术降低Gab2蛋白的表达后SGC7901细胞侵袭转移能力降低，同时E-cadherin表达增多，N-cadherin和MMP-9蛋白表达降低。结论 Gab2可能通过调节EMT在胃癌侵袭转移中发挥重要作用
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