%0 Journal Article
%T Gab2通过调节EMT促进胃癌的侵袭转移
%A 史立宏
%A 姜迎宵
%A 孙秀宁
%A 张宝刚
%A 张小茜
%A 王淑晓
%A 郑书贤
%A 陈美玲
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.0881
%X 摘要 目的 探讨Gab2在胃癌组织中的表达情况及其对胃癌细胞侵袭转移能力的影响与机制。方法 采用免疫组织化学SP法检测胃癌组织中Gab2、E-cadherin、N-cadherin及MMP-9的表达并分析其与淋巴结转移的关系。应用小RNA干扰技术，转染SGC7901细胞株，RT-PCR法检测瞬时转染后Gab2基因表达情况，体外侵袭实验检测细胞转染后侵袭能力的变化，Western blot法检测各蛋白的表达。结果 胃癌组织中Gab2的表达水平与淋巴结转移显著相关（P=0.002），与E-cadherin的表达负相关（r=-0.693, P=0.000），而与N-cadherin和MMP-9的表达水平正相关（r=0.407, P=0.021; r=0.335, P=0.017）。小RNA干扰技术降低Gab2蛋白的表达后SGC7901细胞侵袭转移能力降低，同时E-cadherin表达增多，N-cadherin和MMP-9蛋白表达降低。结论 Gab2可能通过调节EMT在胃癌侵袭转移中发挥重要作用
%K 胃癌
%K SGC7901
%K Gab2
%K 上皮间质转化
%K MMP-9
%K 侵袭
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%U http://www.zlfzyj.com/CN/abstract/abstract9171.shtml