组蛋白去乙酰化酶抑制剂SAHA与紫杉醇联用诱导宫颈癌HeLa细胞凋亡的实验
DOI: 10.3971/j.issn.1000-8578.2018.17.0562
Keywords: 宫颈癌,HeLa细胞,SAHA,紫杉醇,细胞凋亡,Blockade of Radiation-induced EGFR Nuclear Transport Enhances Radiosensitivity of Human Cervical Cancer,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Comparison of Survival and Postoperative Complication of Stage Ⅰb2-Ⅱa2 Cervical Cancer Patients Among Three Kinds of Therapeutic Regimens,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,Effects of Long Non-coding RNA-MALAT1 on Proliferation and Apoptosis of Prostate Cancer Cells,Expression and Correlation of miR-135a-5p, GATA3 and STAT3 in Cervical Cancer,Effect of STAT1 on Cell Cycle of Human Glioma U251 Cells and Related Mechanism,Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer,Indocyanine Green Angiography for Preserving Ureteral Branch of Uterine Artery in Radical Hysterectomy,Metadherin-mediated Cell Proliferation and Paclitaxel Resistance in Nasopharyngeal Carcinoma,Effect of Overexpression of Follistatin-like Protein 1 on Downstream Genes in Cervical Cancer and Bioinformatic Analysis of Downstream Genes,Risk Factors of Lymph Node Metastasis in Early-stage Cervical Cancer Patients and Build of A Nomogram Prediction Model,Effect of SCD1 Inhibitor on Proliferation and Apoptosis of Breast Cancer Cells and Related Mechanism,miR-198 Targets MAPK1 to Regulate Proliferation, Apoptosis and Invasion of Cervical Cancer HeLa Cells
Abstract:
摘要 目的 探讨组蛋白乙酰化抑制剂SAHA联合紫杉醇体外对宫颈癌HeLa细胞增殖抑制效果及其机制。方法 分别设置空白对照组SAHA（10 μmol/L）组、紫杉醇（10 nmol/L）组、SAHA（10 μmol/L）+紫杉醇（10 nmol/L）联合组，采用MTT法检测各组HeLa细胞的抑制率，采用流式细胞术检测HeLa细胞凋亡情况和细胞周期。结果 MTT结果显示经SAHA组、紫杉醇组和联合组分别处理24、48 h后，与SAHA和紫杉醇组比较，联合组能够显著抑制HeLa细胞的增殖，差异有统计学意义，且二者具有叠加作用（Q=0.861, Q=1.25）； HeLa细胞的凋亡实验结果显示联合组的凋亡率分别高于紫杉醇组、SAHA组；HeLa细胞周期实验发现：联合组处理后的HeLa细胞主要处于G0/G1期，提示SAHA与紫杉醇联合使用能够抑制HeLa细胞有丝分裂过程中的DNA合成和复制。结论 SAHA与紫衫醇联用能够抑制宫颈癌HeLa细胞增殖，增强诱导HeLa细胞凋亡的能力，阻滞细胞周期，最终增强抗肿瘤的能力
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