%0 Journal Article
%T 组蛋白去乙酰化酶抑制剂SAHA与紫杉醇联用诱导宫颈癌HeLa细胞凋亡的实验
%A 刘好
%A 张可
%A 张蕾
%A 王晓梅
%A 赵滢滢
%A 金平
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.0562
%X 摘要 目的 探讨组蛋白乙酰化抑制剂SAHA联合紫杉醇体外对宫颈癌HeLa细胞增殖抑制效果及其机制。方法 分别设置空白对照组SAHA（10 μmol/L）组、紫杉醇（10 nmol/L）组、SAHA（10 μmol/L）+紫杉醇（10 nmol/L）联合组，采用MTT法检测各组HeLa细胞的抑制率，采用流式细胞术检测HeLa细胞凋亡情况和细胞周期。结果 MTT结果显示经SAHA组、紫杉醇组和联合组分别处理24、48 h后，与SAHA和紫杉醇组比较，联合组能够显著抑制HeLa细胞的增殖，差异有统计学意义，且二者具有叠加作用（Q=0.861, Q=1.25）； HeLa细胞的凋亡实验结果显示联合组的凋亡率分别高于紫杉醇组、SAHA组；HeLa细胞周期实验发现：联合组处理后的HeLa细胞主要处于G0/G1期，提示SAHA与紫杉醇联合使用能够抑制HeLa细胞有丝分裂过程中的DNA合成和复制。结论 SAHA与紫衫醇联用能够抑制宫颈癌HeLa细胞增殖，增强诱导HeLa细胞凋亡的能力，阻滞细胞周期，最终增强抗肿瘤的能力
%K 宫颈癌
%K HeLa细胞
%K SAHA
%K 紫杉醇
%K 细胞凋亡
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%U http://www.zlfzyj.com/CN/abstract/abstract9169.shtml