NRP-1过表达对乳腺癌细胞MDA-MB-231、SK-BR-3生物学行为的影响
DOI: 10.3971/j.issn.1000-8578.2016.08.006
Keywords: 神经纤毛蛋白-1,乳腺癌,MDA-MB-231,SK-BR-3,Multi-factors Affect Cardiac Toxicity in Radiotherapy on Left-sided Breast Cancer,Effect of Neoadjuvant Chemotherapy Cycles on Pathologic Complete Response Rate Under Different Estrogen Receptor Status of Breast Cancer,Review of Targeted Therapy for Breast Cancer with BRCA Genes Mutation,Autophagic Cell Death Induced by GANT61 Inhibits Proliferation of Human Breast Cancer Cell Line MCF-7,Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88,Progress on FOXP3 Expression in Breast Cancer Cells,Effect of Hormones from Adrenal Gland on Breast Cancer Metastasis in Mice,Effect of SAHA on Proliferation and Apoptosis of Breast Cancer Cells MDA-MB-435 and Related Mechanism,Analysis E-cad and VEGF Expression in Triple-negative Breast Cancer of Han and Uygur,Regulations and Roles of Breast Cancer Stem Cells,Prediction of Disease Specific Survival Rates by Genes Associated with Relapse of Breast Cancer,Correlation Between Tumor Associated Macrophages and CXCL12 Expression in Different Breast Tissues,Effect of Operation Incision Selection on Success rate of Sentinel Lymph Node Biopsy and Subcutaneous Hydrops in Breast Cancer,Expression and Clinical Significance of Artemin Protein in Triple-negative and Nontriple-negative Breast Cancer Tissues,Value of 21-gene Assay in Treatment Decisions for Hormone Receptor-positive Nodepositive Early Breast Cancer
Abstract:
摘要 目的 通过上调乳腺癌细胞MDA-MB-231、SK-BR-3中NRP-1的表达，观察NRP-1对细胞增殖、凋亡、迁移及侵袭能力的影响。方法 构建pcDNA3.1-NRP-1表达载体，脂质体介导NRP-1 表达质粒转染MDA-MB-231、SK-BR-3细胞，用G418筛选出稳定转染的乳腺癌细胞株。利用RTqPCR、Western blot法分别检测NRP-1基因mRNA及其蛋白表达；CCK-8法、AnnexinⅤ-APC/7-AAD法、Transwell小室分别检测转染细胞增殖率、凋亡率及侵袭、迁移能力。结果 成功构建pcDNA3.1- NRP-1表达载体，转染MDA-MB-231、SK-BR-3细胞并筛选稳定表达系。与对照组相比，过表达组细胞的NRP-1 mRNA及蛋白表达水平明显升高（均P<0.05）；NRP-1过表达组的细胞较对照组增殖率增加、凋亡率降低、侵袭及迁移能力增强（均P<0.05)。结论 NRP-1在乳腺癌发展、浸润、转移中起着一定的作用，它可促进乳腺癌细胞增殖、迁移和侵袭，抑制其凋亡
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