%0 Journal Article
%T NRP-1过表达对乳腺癌细胞MDA-MB-231、SK-BR-3生物学行为的影响
%A 张梦瑾
%A 徐杰
%A 杜秀平
%A 王红梅
%A 韩正祥
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.08.006
%X 摘要 目的 通过上调乳腺癌细胞MDA-MB-231、SK-BR-3中NRP-1的表达，观察NRP-1对细胞增殖、凋亡、迁移及侵袭能力的影响。方法 构建pcDNA3.1-NRP-1表达载体，脂质体介导NRP-1 表达质粒转染MDA-MB-231、SK-BR-3细胞，用G418筛选出稳定转染的乳腺癌细胞株。利用RTqPCR、Western blot法分别检测NRP-1基因mRNA及其蛋白表达；CCK-8法、AnnexinⅤ-APC/7-AAD法、Transwell小室分别检测转染细胞增殖率、凋亡率及侵袭、迁移能力。结果 成功构建pcDNA3.1- NRP-1表达载体，转染MDA-MB-231、SK-BR-3细胞并筛选稳定表达系。与对照组相比，过表达组细胞的NRP-1 mRNA及蛋白表达水平明显升高（均P<0.05）；NRP-1过表达组的细胞较对照组增殖率增加、凋亡率降低、侵袭及迁移能力增强（均P<0.05)。结论 NRP-1在乳腺癌发展、浸润、转移中起着一定的作用，它可促进乳腺癌细胞增殖、迁移和侵袭，抑制其凋亡
%K 神经纤毛蛋白-1
%K 乳腺癌
%K MDA-MB-231
%K SK-BR-3
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%U http://www.zlfzyj.com/CN/abstract/abstract8812.shtml