宫颈癌细胞中ΔNp63α的MicroRNA表达谱及其对hsa-let-7b-3p的调控机制
DOI: 10.3971/j.issn.1000-8578.2018.17.0542
Keywords: Δ N p 6 3 α,子宫颈癌,miRNA,hsa-let-7b-3p,增殖,凋亡,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Autophagy Related Protein 5 Regulates Colon Cancer Cell Senescence Through p53/p21 Pathway,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism,Downregulation of Skp2 Expression Inhibits Malignant Phenotype of Osteosarcoma U2OS Cells,Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition,Effects of NDUFA4 Overexpression on Growth of Human Colon Cancer Cells in vitro
Abstract:
摘要 目的 在宫颈鳞癌细胞中筛选出ΔNp63α显著调控的miRNAs，并探讨其对宫颈癌细胞功能的影响。 方法 通过miRNA芯片技术筛选出ΔNp63α过表达的SiHa细胞株（SiHa/p63）中差异明显的miRNAs，并进行qRT-PCR验证；通过qRT-PCR在沉默ΔNp63α的ME-180细胞株（ME-180/Shp63）中检测差异表达的miRNAs，并选择差异较显著的hsa-let-7b-3p作为研究对象；向SiHa细胞中转染hsa-let-7b-3p 刺激剂，通过生长曲线、Western blot以及流式细胞仪检测hsa-let-7b-3p对SiHa细胞增殖及凋亡的影响。 结果 （1）在ΔNp63α过表达的SiHa细胞株中，筛选出10个变化较显著的miRNAs；（2）在ΔNp63α沉默的ME-180细胞中，筛选出5个与上述变化趋势一致的miRNAs；（3）hsa-let-7b-3p过表达抑制SiHa细胞的增殖；（4）hsa-let-7b-3p过表达促进SiHa细胞的凋亡。结论 宫颈癌细胞株中，miRNA hsalet-7b-3p的表达与ΔNp63α的表达呈正相关，且可以有效抑制细胞增殖，其作用机制可能与诱导细胞周期阻滞和促进细胞凋亡相关
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