%0 Journal Article
%T 宫颈癌细胞中ΔNp63α的MicroRNA表达谱及其对hsa-let-7b-3p的调控机制
%A 吴大保
%A 周颖
%A 宋伟国
%A 朱靖
%A 申震
%A 程勇
%A 肖卫华
%A 钱莉莉
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.0542
%X 摘要 目的 在宫颈鳞癌细胞中筛选出ΔNp63α显著调控的miRNAs，并探讨其对宫颈癌细胞功能的影响。 方法 通过miRNA芯片技术筛选出ΔNp63α过表达的SiHa细胞株（SiHa/p63）中差异明显的miRNAs，并进行qRT-PCR验证；通过qRT-PCR在沉默ΔNp63α的ME-180细胞株（ME-180/Shp63）中检测差异表达的miRNAs，并选择差异较显著的hsa-let-7b-3p作为研究对象；向SiHa细胞中转染hsa-let-7b-3p 刺激剂，通过生长曲线、Western blot以及流式细胞仪检测hsa-let-7b-3p对SiHa细胞增殖及凋亡的影响。 结果 （1）在ΔNp63α过表达的SiHa细胞株中，筛选出10个变化较显著的miRNAs；（2）在ΔNp63α沉默的ME-180细胞中，筛选出5个与上述变化趋势一致的miRNAs；（3）hsa-let-7b-3p过表达抑制SiHa细胞的增殖；（4）hsa-let-7b-3p过表达促进SiHa细胞的凋亡。结论 宫颈癌细胞株中，miRNA hsalet-7b-3p的表达与ΔNp63α的表达呈正相关，且可以有效抑制细胞增殖，其作用机制可能与诱导细胞周期阻滞和促进细胞凋亡相关
%K Δ N p 6 3 α
%K 子宫颈癌
%K miRNA
%K hsa-let-7b-3p
%K 增殖
%K 凋亡
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%U http://www.zlfzyj.com/CN/abstract/abstract9119.shtml