miR-130b调控RAB34的表达在胰腺癌发生发展中的作用
DOI: 10.3971/j.issn.1000-8578.2018.17.0960
Keywords: One Case Report,Relationship of Para-aortic Lymph Nodes (PALN) Status with Prognosis and Clinicopathological Features of Pancreatic Cancer Patients After Pancreatectomy,Effects of Metformin on ALDH1 Expression in Pancreatic Cancer and Pancreatic Cancer Stem Cells,Effect of FDG-PET/CT in Diagnosis, Staging and Prognosis of Pancreatic Cancer: A Meta-analysis,Efficacy of IMRT Combined with Capecitabine Concurrent Chemotherapy and High Intensity Focused Ultrasound for Elderly Patients with Non-operatable Pancreatic Cancer,Progress of miRNAs Related to Pancreatic Cancer,Clinical Analysis of Multimodality Treatments for Pancreatic Cancer Patients with Liver Metastases,Effect of ABCG2 Expression on Chemoresistance of Pancreatic Carcinoma Cell Line#br# SW1990,Review on Low-molecular-weight Heparin in Improving Prognosis of Pancreatic Cancer
Abstract:
摘要 目的 探讨miR-130b调控Ras相关蛋白34（Ras-related proteins in brain 34, RAB34）在胰腺癌中的表达。方法 收集22例胰腺癌及其癌旁组织标本，采用免疫组织化学、Western blot和qRT-PCR法检测RAB34的表达；qRT-PCR法检测miR-130b表达；细胞转染法检测miR-130b调控RAB34表达，双荧光素酶报告基因检测系统验证miR-130b结合RAB34的3’UTR的靶位点。结果 与癌旁组织对比，RAB34在胰腺癌组织中显著高表达，且miR-130b显著低表达；转染miR-130b mimics可抑制RAB34的表达；RAB34是miR-130b作用的靶基因。结论 RAB34是胰腺癌的分子标志物，miR-130b可调控其表达
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