低糖增强肿瘤对二甲双胍的敏感度
DOI: 10.3971/j.issn.1000-8578.2018.18.0189
Keywords: A Scientometric Analysis,A Case Report of Four Primary Malignant Tumors,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Dedifferentiated Liposarcoma of Testis: A Case Report and Literature Review,G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性,Diffusion-weighted Magnetic Resonance Imaging Evaluates Salivary Gland Function Damage in Nasopharyngeal Carcinoma Patients Treated with Intensity-modulated Radiation Therapy,Research Advances on Effect of Adipose-derived Stem Cells on Biological Characteristics of Breast Cancer,Advances of 125I Radioactive Particle Chain Intracavitary Brachytherapy,Expression of Infiltrating NK Cell Surface Receptors in Peripheral Blood and Cancer Tissues of Patients with Esophageal Squamous Cell Carcinoma,Risk Factors of Pathological Upstaging for Patients with Clinical T2N0M0 Esophageal Squamous Cell Carcinoma,Progress of Stimulation Response Strategy Based on Nanomaterials in Tumor Therapy,Advances in Molecular Mechanisms of Early Bone Metastasis,Diagnostic Value of Peripheral Blood NLR and PLR in Gastrointestinal Pancreatic Neuroendocrine Neoplasm
Abstract:
摘要 目的 观察不同浓度葡萄糖对二甲双胍抗肿瘤作用的影响并探讨其作用机制。方法 在含不同浓度葡萄糖的培养基中加入二甲双胍培养细胞24 h后，检测细胞增殖情况及ATP水平。构建裸鼠皮下移植瘤，随机分成四组：正常饮食（SD）组、SD+二甲双胍（Met）组、生酮饮食（KD）组、KD+Met组，实验干预8周后，检测瘤体体积、重量以及裸鼠血糖水平。结果 培养基中葡萄糖水平≥10 mmol/L时，二甲双胍对胰腺癌细胞增殖抑制不明显，但在低糖培养基（≤5 mmol/L）中， 10mmol/L二甲双胍能明显抑制胰腺癌细胞增殖。同时，低糖培养基中各细胞ATP水平显著降低（P<0.01）。体内实验中，KD组血糖水平明显降低（P<0.01），肿瘤生长受到显著抑制（P<0.01）。与KD组相比，KD+Met组可进一步抑制移植瘤生长（P<0.05）。结论 低糖增强肿瘤对二甲双胍的敏感度，其机制可能与减少机体ATP生成有关
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