%0 Journal Article
%T 低糖增强肿瘤对二甲双胍的敏感度
%A 丛明华
%A 俞伟男
%A 张杰
%A 徐嘉溪
%A 石汉平
%A 简美诚
%A 缪明永
%A 高云
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.18.0189
%X 摘要 目的 观察不同浓度葡萄糖对二甲双胍抗肿瘤作用的影响并探讨其作用机制。方法 在含不同浓度葡萄糖的培养基中加入二甲双胍培养细胞24 h后，检测细胞增殖情况及ATP水平。构建裸鼠皮下移植瘤，随机分成四组：正常饮食（SD）组、SD+二甲双胍（Met）组、生酮饮食（KD）组、KD+Met组，实验干预8周后，检测瘤体体积、重量以及裸鼠血糖水平。结果 培养基中葡萄糖水平≥10 mmol/L时，二甲双胍对胰腺癌细胞增殖抑制不明显，但在低糖培养基（≤5 mmol/L）中， 10mmol/L二甲双胍能明显抑制胰腺癌细胞增殖。同时，低糖培养基中各细胞ATP水平显著降低（P<0.01）。体内实验中，KD组血糖水平明显降低（P<0.01），肿瘤生长受到显著抑制（P<0.01）。与KD组相比，KD+Met组可进一步抑制移植瘤生长（P<0.05）。结论 低糖增强肿瘤对二甲双胍的敏感度，其机制可能与减少机体ATP生成有关
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%U http://www.zlfzyj.com/CN/abstract/abstract9117.shtml