桔梗皂苷D对人移行细胞癌5637细胞株的诱导凋亡作用及其分子机制
DOI: 10.3971/j.issn.1000-8578.2018.17.1515
Keywords: 桔梗皂苷D,移行细胞癌,5637细胞,凋亡,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism,Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition,Effects of Long Non-coding RNA-MALAT1 on Proliferation and Apoptosis of Prostate Cancer Cells,Expression and Biological Functions of Sestrin2 in Nasopharyngeal Carcinoma,RNA PolymeraseⅠInhibitor Regulates Proliferation, Invasion and Apoptosis of Nasopharyngeal Carcinoma Cell Line CNE-1 by NF-κb,Knockdown of EN2 Induces Apoptosis and Enhances PTEN Protein Expression in Hepatocellular Carcinoma Cells,Effect of TAK-242 on Proliferation and Apoptosis of Human Multiple Myeloma Cells,Effect of STAT1 on Cell Cycle of Human Glioma U251 Cells and Related Mechanism,Role of Gadd45 Protein Family in Tumorigenesis and Development
Abstract:
摘要 目的 探讨桔梗皂苷D对人移行细胞癌5637细胞诱导凋亡作用及分子机制。方法 HE染色和透射电子显微镜观察细胞的形态学变化；流式细胞学技术检测细胞凋亡率的变化；Western blot法检测凋亡通路相关蛋白的表达变化；PCR法检测p53、Bcl-2、Bax mRNA表达；免疫组织化学法检测凋亡相关蛋白表达的影响。结果 通过HE染色和电子显微镜观察桔梗皂苷D组5637细胞，可见凋亡细胞，凋亡指数明显高于对照组（P<0.01）；与对照组细胞相比，桔梗皂苷D组5637细胞Survivin、Livin表达量明显下降（P<0.05）；Caspase-9、Caspase-8、Caspase-3、Cyt-c表达量与对照组比较，明显增加（P<0.05）；p53、Bax mRNA水平明显升高，Bcl-2 mRNA表达下降（P<0.01）； Bax、Caspase-9、Caspase-8以及Caspase-3表达较对照组增多，而Bcl-2表达则明显下降（P<0.01）。结论 桔梗皂苷D能诱导人移行细胞癌5637细胞凋亡，其作用机制可能与上调Caspase-9、Caspase-8、Caspase-3、Cyt-c、p53和下调Bcl-2表达、激活线粒体途径和死亡受体途径有关
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