%0 Journal Article
%T 桔梗皂苷D对人移行细胞癌5637细胞株的诱导凋亡作用及其分子机制
%A 孙抒
%A 李晓刚
%A 杨万山
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.1515
%X 摘要 目的 探讨桔梗皂苷D对人移行细胞癌5637细胞诱导凋亡作用及分子机制。方法 HE染色和透射电子显微镜观察细胞的形态学变化；流式细胞学技术检测细胞凋亡率的变化；Western blot法检测凋亡通路相关蛋白的表达变化；PCR法检测p53、Bcl-2、Bax mRNA表达；免疫组织化学法检测凋亡相关蛋白表达的影响。结果 通过HE染色和电子显微镜观察桔梗皂苷D组5637细胞，可见凋亡细胞，凋亡指数明显高于对照组（P<0.01）；与对照组细胞相比，桔梗皂苷D组5637细胞Survivin、Livin表达量明显下降（P<0.05）；Caspase-9、Caspase-8、Caspase-3、Cyt-c表达量与对照组比较，明显增加（P<0.05）；p53、Bax mRNA水平明显升高，Bcl-2 mRNA表达下降（P<0.01）； Bax、Caspase-9、Caspase-8以及Caspase-3表达较对照组增多，而Bcl-2表达则明显下降（P<0.01）。结论 桔梗皂苷D能诱导人移行细胞癌5637细胞凋亡，其作用机制可能与上调Caspase-9、Caspase-8、Caspase-3、Cyt-c、p53和下调Bcl-2表达、激活线粒体途径和死亡受体途径有关
%K 桔梗皂苷D
%K 移行细胞癌
%K 5637细胞
%K 凋亡
%K Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer
%K Effect of ANLN Gene Silencing on Invasion
%K Migration
%K Proliferation and Apoptosis of Gastric Cancer Cells
%K Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro
%K Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells
%K Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line
%K Effects of Pachymaran on Proliferation
%K Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism
%K Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism
%K Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition
%K Effects of Long Non-coding RNA-MALAT1 on Proliferation and Apoptosis of Prostate Cancer Cells
%K Expression and Biological Functions of Sestrin2 in Nasopharyngeal Carcinoma
%K RNA PolymeraseⅠInhibitor Regulates Proliferation
%K Invasion and Apoptosis of Nasopharyngeal Carcinoma Cell Line CNE-1 by NF-κb
%K Knockdown of EN2 Induces Apoptosis and Enhances PTEN Protein Expression in Hepatocellular Carcinoma Cells
%K Effect of TAK-242 on Proliferation and Apoptosis of Human Multiple Myeloma Cells
%K Effect of STAT1 on Cell Cycle of Human Glioma U251 Cells and Related Mechanism
%K Role of Gadd45 Protein Family in Tumorigenesis and Development
%U http://www.zlfzyj.com/CN/abstract/abstract9225.shtml