神经节苷脂GM3对人宫颈癌HeLa细胞凋亡与血管内皮生长因子表达的影响
DOI: 10.3971/j.issn.1000-8578.2015.05.006
Keywords: 神经节苷脂GM3,HeLa细胞,细胞增殖,凋亡,血管内皮生长因子,Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells,Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88,siRNA-mediated Inhibition of MTH1 Gene Expression Promotes Apoptosis and Reduces Migration of Hepatocellular Carcinoma HepG2 Cells,Effect of SAHA on Proliferation and Apoptosis of Breast Cancer Cells MDA-MB-435 and Related Mechanism,Analysis E-cad and VEGF Expression in Triple-negative Breast Cancer of Han and Uygur,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism,Progress of Relationships of Calprotectin with Tumours,Experimental Investigation on Apoptosis of Cervical Cancer Cells Promoted by NF-κB Decoy ODN,Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation,MDM2 siRNA Inhibits Angiogenesis by Reducing Vascular Endothelial Growth Factor Production in Breast Cancer Cells,Expressions of MMP-7 mRNA, sMICA, VEGF in Peripheral Blood of Lung Cancer Patients and Their Relationships with Invasion and Metastasis,Expression of Toll-like Receptor 4 in Lymphoma Cell Lines and Its Effect on Cells Proliferation and Drug Resistance,Research Advances of ASPP in Regulating Apoptosis of Tumor Cells,Periplocin Extracted from Cortex Periplocae(CPP) Induced Apoptosis of Gastric Cancer Cells MGC-803 Through Lysosomal Pathway
Abstract:
摘要 目的 研究外源性神经节苷脂GM3对人宫颈癌HeLa细胞增殖、凋亡和VEGF表达的影响。方法 不同浓度GM3干预HeLa细胞24 h后，MTT法检测细胞增殖变化，流式细胞学技术检测细胞凋亡率，RT-PCR技术检测细胞VEGF mRNA表达水平，Western blot技术检测细胞VEGF蛋白水平。结果（1）GM3浓度分别为2 、10、50和250 μmol/L时，HeLa细胞生长抑制率分别为9.8%、32.9%、50.7%和78.6%，半数抑制浓度（IC50）为49.8 μmol/L。（2）GM3浓度分别为10、50和250 μmol/L时，HeLa细胞凋亡率分别为（4.9±0.4）%、（15.1±0.3）%、（24.4±0.7）%。（3）当GM3浓度高于10 μmol/L时，HeLa细胞VEGF mRNA表达水平以及VEGF蛋白表达水平均显著下降（P<0.05）。结论 外源性神经节苷脂GM3能呈浓度依赖性抑制人宫颈癌HeLa细胞增殖，促进HeLa细胞凋亡，下调HeLa VEGF mRNA水平和蛋白水平，提示GM3可能通过调节肿瘤细胞凋亡和肿瘤血管生成而发挥双重抗肿瘤作用
Full-Text
