%0 Journal Article
%T 神经节苷脂GM3对人宫颈癌HeLa细胞凋亡与血管内皮生长因子表达的影响
%A 伍钢
%A 周毅
%A 朱燕
%A 朱芳
%A 欧阳礼辰
%A 陈静
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.05.006
%X 摘要 目的 研究外源性神经节苷脂GM3对人宫颈癌HeLa细胞增殖、凋亡和VEGF表达的影响。方法 不同浓度GM3干预HeLa细胞24 h后，MTT法检测细胞增殖变化，流式细胞学技术检测细胞凋亡率，RT-PCR技术检测细胞VEGF mRNA表达水平，Western blot技术检测细胞VEGF蛋白水平。结果（1）GM3浓度分别为2 、10、50和250 μmol/L时，HeLa细胞生长抑制率分别为9.8%、32.9%、50.7%和78.6%，半数抑制浓度（IC50）为49.8 μmol/L。（2）GM3浓度分别为10、50和250 μmol/L时，HeLa细胞凋亡率分别为（4.9±0.4）%、（15.1±0.3）%、（24.4±0.7）%。（3）当GM3浓度高于10 μmol/L时，HeLa细胞VEGF mRNA表达水平以及VEGF蛋白表达水平均显著下降（P<0.05）。结论 外源性神经节苷脂GM3能呈浓度依赖性抑制人宫颈癌HeLa细胞增殖，促进HeLa细胞凋亡，下调HeLa VEGF mRNA水平和蛋白水平，提示GM3可能通过调节肿瘤细胞凋亡和肿瘤血管生成而发挥双重抗肿瘤作用
%K 神经节苷脂GM3
%K HeLa细胞
%K 细胞增殖
%K 凋亡
%K 血管内皮生长因子
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%U http://www.zlfzyj.com/CN/abstract/abstract8486.shtml