出核因子CRM1抑制剂KPT-185对人前列腺癌细胞生长的影响
DOI: 10.3971/j.issn.1000-8578.2015.07.008
Keywords: A Metaanalysis,Effect of Intensity Modulated Radiotherapy Combined with Hormonal Therapy on Fatigue in Patients with Locally Advanced Prostate Cancer,Effect of Long Non-coding RNA LOC100294362 on Proliferation and Invasion of Breast Cancer Cells,Research Advances of ASPP in Regulating Apoptosis of Tumor Cells,Nitric Oxide-donor Drugs and Prostate Cancer: An Update,Valuation of SUVmax, Ki-67, p53 and EGFR in Predicting Effect of Neoadjuvant Chemotherapy on Triple-negative Breast Cancer Patients,Effects of PPP2R5C Downregulation on Expression Profile of GSK-3β, MDM2, pTEN and TP53 Signal Pathway Regulating Genes in Jurkat Cells,Role of p53β Isoform in Inhibitory Effect of rmhTNF Combined with Cisplatin on Human Gastric Cancer Cell Growth,Comparison of Clinical Value of Transrectal Ultrasound, Contrast-enhanced Ultrasonography and Dynamic Contrast Material-enhanced Magnetic Resonance Imaging for Detecting Prostate Carcinoma,Clinical Observation of Abriaterone Acetate and Prednison on Patients with Castrationresistant Prostate Cancer,Inhibitory Effect of Sorafenib on Growth of Human Esophageal Cancer Cells EC9706,Effect of Tetrandrine on Proliferation and Apoptosis of Prostate Cancer Cells PC3,Laser Interstitial Thermotherapy in Treatment of Prostate Cancer
Abstract:
摘要 目的 探讨出核因子（chromosome maintenance region 1, CRM1）抑制剂KPT-185对人前列腺癌细胞生长、凋亡、迁徙的影响及影响产生的原因。方法 采用MTT、细胞集落形成能力测定法检测KPT-185对前列腺癌细胞生长的影响；划痕愈合实验检测KPT-185对前列腺癌细胞迁徙能力的影响；流式细胞仪检测KPT-185对细胞周期的影响；人组蛋白（Histone）/DNA 酶联免疫法和膜联蛋白V-FITC/PI双染法流式细胞术检测细胞凋亡；免疫印迹检测蛋白表达。结果 KPT-185能极大地抑制人前列腺癌细胞的生长，使其细胞周期阻滞于G0~G1期，并导致细胞凋亡。KPT-185引起的细胞生长抑制和凋亡是通过其抑制CRM1的核输出，使抑癌蛋白p53 和p21保留于核内而发挥作用的。结论 KPT-185是一种新型的CRM1抑制剂，有望成为治疗前列腺癌的候选药物
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