%0 Journal Article
%T 出核因子CRM1抑制剂KPT-185对人前列腺癌细胞生长的影响
%A 唐旭
%A 曾彬
%A 高剑坤
%A 龙波
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.07.008
%X 摘要 目的 探讨出核因子（chromosome maintenance region 1, CRM1）抑制剂KPT-185对人前列腺癌细胞生长、凋亡、迁徙的影响及影响产生的原因。方法 采用MTT、细胞集落形成能力测定法检测KPT-185对前列腺癌细胞生长的影响；划痕愈合实验检测KPT-185对前列腺癌细胞迁徙能力的影响；流式细胞仪检测KPT-185对细胞周期的影响；人组蛋白（Histone）/DNA 酶联免疫法和膜联蛋白V-FITC/PI双染法流式细胞术检测细胞凋亡；免疫印迹检测蛋白表达。结果 KPT-185能极大地抑制人前列腺癌细胞的生长，使其细胞周期阻滞于G0~G1期，并导致细胞凋亡。KPT-185引起的细胞生长抑制和凋亡是通过其抑制CRM1的核输出，使抑癌蛋白p53 和p21保留于核内而发挥作用的。结论 KPT-185是一种新型的CRM1抑制剂，有望成为治疗前列腺癌的候选药物
%K A Metaanalysis
%K Effect of Intensity Modulated Radiotherapy Combined with Hormonal Therapy on Fatigue in Patients with Locally Advanced Prostate Cancer
%K Effect of Long Non-coding RNA LOC100294362 on Proliferation and Invasion of Breast Cancer Cells
%K Research Advances of ASPP in Regulating Apoptosis of Tumor Cells
%K Nitric Oxide-donor Drugs and Prostate Cancer: An Update
%K Valuation of SUVmax
%K Ki-67
%K p53 and EGFR in Predicting Effect of Neoadjuvant Chemotherapy on Triple-negative Breast Cancer Patients
%K Effects of PPP2R5C Downregulation on Expression Profile of GSK-3β
%K MDM2
%K pTEN and TP53 Signal Pathway Regulating Genes in Jurkat Cells
%K Role of p53β Isoform in Inhibitory Effect of rmhTNF Combined with Cisplatin on Human Gastric Cancer Cell Growth
%K Comparison of Clinical Value of Transrectal Ultrasound
%K Contrast-enhanced Ultrasonography and Dynamic Contrast Material-enhanced Magnetic Resonance Imaging for Detecting Prostate Carcinoma
%K Clinical Observation of Abriaterone Acetate and Prednison on Patients with Castrationresistant Prostate Cancer
%K Inhibitory Effect of Sorafenib on Growth of Human Esophageal Cancer Cells EC9706
%K Effect of Tetrandrine on Proliferation and Apoptosis of Prostate Cancer Cells PC3
%K Laser Interstitial Thermotherapy in Treatment of Prostate Cancer
%U http://www.zlfzyj.com/CN/abstract/abstract8537.shtml