成纤维细胞系3T3细胞来源exosome对小鼠乳腺癌细胞增殖能力的影响
DOI: 10.3971/j.issn.1000-8578.2016.01.003
Keywords: 外泌小体,乳腺癌,增殖,表皮生长因子受体2,赫赛汀,Multi-factors Affect Cardiac Toxicity in Radiotherapy on Left-sided Breast Cancer,Effect of Neoadjuvant Chemotherapy Cycles on Pathologic Complete Response Rate Under Different Estrogen Receptor Status of Breast Cancer,Review of Targeted Therapy for Breast Cancer with BRCA Genes Mutation,Autophagic Cell Death Induced by GANT61 Inhibits Proliferation of Human Breast Cancer Cell Line MCF-7,Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells,Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88,Progress on FOXP3 Expression in Breast Cancer Cells,Effect of Hormones from Adrenal Gland on Breast Cancer Metastasis in Mice,Effect of SAHA on Proliferation and Apoptosis of Breast Cancer Cells MDA-MB-435 and Related Mechanism,Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells,Effect of RORα Overexpression on Proliferation, Migration and Invasion of Human Gastric MGC803 Cells,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Analysis E-cad and VEGF Expression in Triple-negative Breast Cancer of Han and Uygur,Regulations and Roles of Breast Cancer Stem Cells,Prediction of Disease Specific Survival Rates by Genes Associated with Relapse of Breast Cancer
Abstract:
摘要 目的 观察小鼠成纤维细胞系3T3来源的外泌小体（exosome）对小鼠乳腺癌细胞4T1增殖能力的影响，并探索其中可能的机制。方法 PureExo Exosome提取试剂盒提取3T3细胞上清液中的exosome，按照不同浓度及时间作用于4T1细胞，CCK8法检测4T1细胞的增殖能力，BrdU/PI双掺入法测定细胞DNA合成及细胞周期；免疫印迹法（Western blot）及荧光定量实时PCR（qPCR）检测人表皮生长因子受体2（epidermal growth factor receptor-2, EGFR2，也称HER2）及下游PI3K/AKT信号转导通路相关蛋白的变化。利用HER2单克隆抗体靶向药物赫赛汀（Herceptin），观察exosome是否影响4T1细胞对于Herceptin敏感度。结果 exosome处理组OD450吸光度值显著高于对照组（P<0.05），细胞增殖及细胞周期进程加快。Western blot及qPCR实验提示随着exosome浓度的增加，HER2表达逐渐升高， AKT磷酸化水平增加。而同时给予exosome可明显增加4T1细胞对Herceptin的敏感度。结论 小鼠成纤维细胞系3T3来源exosome可促进小鼠乳腺癌细胞4T1增殖及周期进程，并且可能通过HER2激活其下游PI3K/AKT信号通路发挥上述作用
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