%0 Journal Article
%T 成纤维细胞系3T3细胞来源exosome对小鼠乳腺癌细胞增殖能力的影响
%A 王雅琴
%A 陈智
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.01.003
%X 摘要 目的 观察小鼠成纤维细胞系3T3来源的外泌小体（exosome）对小鼠乳腺癌细胞4T1增殖能力的影响，并探索其中可能的机制。方法 PureExo Exosome提取试剂盒提取3T3细胞上清液中的exosome，按照不同浓度及时间作用于4T1细胞，CCK8法检测4T1细胞的增殖能力，BrdU/PI双掺入法测定细胞DNA合成及细胞周期；免疫印迹法（Western blot）及荧光定量实时PCR（qPCR）检测人表皮生长因子受体2（epidermal growth factor receptor-2, EGFR2，也称HER2）及下游PI3K/AKT信号转导通路相关蛋白的变化。利用HER2单克隆抗体靶向药物赫赛汀（Herceptin），观察exosome是否影响4T1细胞对于Herceptin敏感度。结果 exosome处理组OD450吸光度值显著高于对照组（P<0.05），细胞增殖及细胞周期进程加快。Western blot及qPCR实验提示随着exosome浓度的增加，HER2表达逐渐升高， AKT磷酸化水平增加。而同时给予exosome可明显增加4T1细胞对Herceptin的敏感度。结论 小鼠成纤维细胞系3T3来源exosome可促进小鼠乳腺癌细胞4T1增殖及周期进程，并且可能通过HER2激活其下游PI3K/AKT信号通路发挥上述作用
%K 外泌小体
%K 乳腺癌
%K 增殖
%K 表皮生长因子受体2
%K 赫赛汀
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%U http://www.zlfzyj.com/CN/abstract/abstract8673.shtml