miRNA-302b在结肠癌HT-29细胞“干性”维持中的作用
DOI: 10.3971/j.issn.1000-8578.2017.17.0420
Keywords: Take Paneth Cells as an Example,Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells,Effect of Jinlong Capsule on Vasculogenic Mimicry and Mig-7 of Colon Cancer,Effect of PTTG1 Expression on Invasion and Migration of Colon Cancer Cell SW480 and Its Possible Mechanism,One Case Report of Ablation by Nano Knife on Unresectable Liver Metastases from Colon Cancer,Expression and Clinical Significance of Stem Cells Gene Lgr5 in Esophageal Squamous Cell Carcinoma Tissues,Expressions of MACC1 and c-Met Proteins in Lung Adenocarcinoma and Their Correlation with Postoperative Recurrence,Role of miR-138 in Invasion of Human Colon Cancer Cells SW620 by Regulating PDK1 Expression,Effects of Different Doses of Bevacizumab on Irinotecan Distribution in Nude Mice Bearing Human Colon Cancer Cell Xenografts,Clinical Significance of Different Human mcl1 Subtypes Expression in Colorectal Cancer Tissues,Assessment of Microsatallite Instability in Colorectal Carcinoma: A Comparison Between Immunohistochemistry and PCR Method,NS-398 Reverses Multi-drug Resistance of Colorectal Carcinoma Cells,Plasma miR-21 Expression in Colon Cancer Patients Before and After Operation and Its Clinical Significance,Effects of Aspirin on VEGF, β-catenin Expression in Colon Cancer Cell Lines SW480 and HT-29,Effect of Oxaliplatin on CD44V6, VEGF, survivin, Caspase-3 and Caspase-7 in Dimethylhydrazine-induced Colon Cancer Rats
Abstract:
摘要 目的 探讨miRNA-302b在结肠癌HT-29细胞“干性”维持中的作用及可能机制。方法 体外培养人结肠癌细胞株HT-29，转染miRNA-302b模拟物（miRNA-302b mimics），同时以未转染组（control）和阴性模拟物转染组（negative control-mimics, NC-mimics）作为对照，采用实时定量反转录聚合酶链反应法（real-time PCR）检测HT-29细胞结肠癌“干性”标志物Ascl2、Lgr5、Oct4和Sox2mRNA的表达情况；采用蛋白印迹法（Western blot）检测以上“干性”标志蛋白表达的变化；采用克隆形成实验及干细胞成球实验分别观察HT-29细胞克隆形成和细胞球形成能力的变化。结果 RealtimePCR结果示，miR-302b mimics转染HT-29细胞48 h，“干性”标志物Ascl2、Lgr5、Oct4和Sox2mRNA表达一致性上调（P<0.05）；Western blot结果示Ascl2、Lgr5、Oct4和Sox2蛋白表达也上调（P<0.05）。克隆形成实验及干细胞成球实验结果示，miR-302b mimics转染后HT-29细胞克隆形成和细胞球形成能力增强，与control组和NC-mimics组相比差异均有统计学意义（P<0.05）。结论 miRNA-302b可通过上调“干性”标志物Ascl2、Lgr5、Oct4和Sox2的表达促进HT-29细胞的克隆形成和细胞球形成能力，从而在结肠癌“干性”维持中发挥重要作用
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