%0 Journal Article
%T miRNA-302b在结肠癌HT-29细胞“干性”维持中的作用
%A 俆永素
%A 朱蓉
%A 李伶俐
%A 赵逵
%A 鲁生念
%J 肿瘤防治研究
%D 2017
%R 10.3971/j.issn.1000-8578.2017.17.0420
%X 摘要 目的 探讨miRNA-302b在结肠癌HT-29细胞“干性”维持中的作用及可能机制。方法 体外培养人结肠癌细胞株HT-29，转染miRNA-302b模拟物（miRNA-302b mimics），同时以未转染组（control）和阴性模拟物转染组（negative control-mimics, NC-mimics）作为对照，采用实时定量反转录聚合酶链反应法（real-time PCR）检测HT-29细胞结肠癌“干性”标志物Ascl2、Lgr5、Oct4和Sox2mRNA的表达情况；采用蛋白印迹法（Western blot）检测以上“干性”标志蛋白表达的变化；采用克隆形成实验及干细胞成球实验分别观察HT-29细胞克隆形成和细胞球形成能力的变化。结果 RealtimePCR结果示，miR-302b mimics转染HT-29细胞48 h，“干性”标志物Ascl2、Lgr5、Oct4和Sox2mRNA表达一致性上调（P<0.05）；Western blot结果示Ascl2、Lgr5、Oct4和Sox2蛋白表达也上调（P<0.05）。克隆形成实验及干细胞成球实验结果示，miR-302b mimics转染后HT-29细胞克隆形成和细胞球形成能力增强，与control组和NC-mimics组相比差异均有统计学意义（P<0.05）。结论 miRNA-302b可通过上调“干性”标志物Ascl2、Lgr5、Oct4和Sox2的表达促进HT-29细胞的克隆形成和细胞球形成能力，从而在结肠癌“干性”维持中发挥重要作用
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%U http://www.zlfzyj.com/CN/abstract/abstract9014.shtml