共刺激分子B7-H3在骨肉瘤细胞侵袭转移中的作用及机制
DOI: 10.3971/j.issn.1000-8578.2015.07.001
Keywords: 共刺激分子,B7-H3,骨肉瘤,侵袭,Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells,Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells,Mechanism of Ibuprofen Regulating Migration and Invasion of Liver Cancer QGY-7703 Cells Through PI3K/Akt/mTOR Signaling Pathway,Effects of Silencing B7-H3 Gene on Invasion of Human Hepatocellular Carcinoma Cells,Effect of RORα Overexpression on Proliferation, Migration and Invasion of Human Gastric MGC803 Cells,Effect of PTTG1 Expression on Invasion and Migration of Colon Cancer Cell SW480 and Its Possible Mechanism,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Effect of Ectopic Expression of Transmembrane Adaptor PAG1 on Motility of Cutaneous Squamous Cell Carcinoma A431 Cells in vitro,Effects on Cell Proliferation, Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway,Effect of Rap2a on Invasion and Metastasis of Lung Cancer Cells and Its Related Mechanism,Progress of TBX3 in Development of Malignant Tumors,Effect of Vascular Endothelial Cadherin on Invasion and Metastasis of Non-small Cell Lung Cancer Cells and Related Mechanism,Expressions of MMP-7 mRNA, sMICA, VEGF in Peripheral Blood of Lung Cancer Patients and Their Relationships with Invasion and Metastasis,Effect of Long Non-coding RNA LOC100294362 on Proliferation and Invasion of Breast Cancer Cells,Effect of AT Motif Binding Factor 1 on Proliferation and Invasion of Colorectal Carcinoma Cell Line LOVO
Abstract:
摘要 目的 检测共刺激分子B7-H3在骨肉瘤细胞侵袭转移中的作用及机制。方法 运用RT-PCR（reverse transcription-polymerase chain reaction）和Western blot方法检测B7-H3分子在三种骨肉瘤细胞U-2OS、MG-63及Saos-2中的表达。通过Lipofectamine？ 2000体外转染B7-H3 siRNA或B7-H3 cDNA质粒至骨肉瘤细胞，RT-PCR和Western blot检测细胞中B7-H3 mRNA和蛋白的表达。Transwell侵袭小室实验检测过表达/沉默B7-H3分子对骨肉瘤细胞侵袭能力及对MMP-2表达水平的影响。结果 共刺激分子B7-H3在骨肉瘤细胞中均组成性表达。转染B7-H3 siRNA基因后，MG-63细胞B7-H3基因表达水平显著低于空载体转染组和未转染组 [（0.131±0.002）vs.（0.504±0.007），（0.131±0.002）vs.（0.613±0.013），均P<0.05]。与对照组细胞相比，转染B7-H3 siRNA后MG-63细胞的侵袭力明显下降（P<0.05），基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2）分子的表达水平显著下降（P<0.05）。转染B7-H3 cDNA的Saos-2细胞B7-H3 基因表达水平显著高于空载体转染组和未转染组，[(0.701±0.008) vs. (0.512±0.007)]，[(0.701±0.008) vs. (0.403±0.013)]，均P<0.05]，Saos-2细胞的侵袭力明显增加（P<0.05），MMP-2分子的表达水平显著增加，与对照组相比，差异具有统计学意义（P<0.05）。结论 B7-H3基因可能通过调控MMP-2分子进而参与调节骨肉瘤的侵袭转移
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