%0 Journal Article
%T 共刺激分子B7-H3在骨肉瘤细胞侵袭转移中的作用及机制
%A 张英泽
%A 曹娜娜
%A 王玲
%A 陈伟
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.07.001
%X 摘要 目的 检测共刺激分子B7-H3在骨肉瘤细胞侵袭转移中的作用及机制。方法 运用RT-PCR（reverse transcription-polymerase chain reaction）和Western blot方法检测B7-H3分子在三种骨肉瘤细胞U-2OS、MG-63及Saos-2中的表达。通过Lipofectamine？ 2000体外转染B7-H3 siRNA或B7-H3 cDNA质粒至骨肉瘤细胞，RT-PCR和Western blot检测细胞中B7-H3 mRNA和蛋白的表达。Transwell侵袭小室实验检测过表达/沉默B7-H3分子对骨肉瘤细胞侵袭能力及对MMP-2表达水平的影响。结果 共刺激分子B7-H3在骨肉瘤细胞中均组成性表达。转染B7-H3 siRNA基因后，MG-63细胞B7-H3基因表达水平显著低于空载体转染组和未转染组 [（0.131±0.002）vs.（0.504±0.007），（0.131±0.002）vs.（0.613±0.013），均P<0.05]。与对照组细胞相比，转染B7-H3 siRNA后MG-63细胞的侵袭力明显下降（P<0.05），基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2）分子的表达水平显著下降（P<0.05）。转染B7-H3 cDNA的Saos-2细胞B7-H3 基因表达水平显著高于空载体转染组和未转染组，[(0.701±0.008) vs. (0.512±0.007)]，[(0.701±0.008) vs. (0.403±0.013)]，均P<0.05]，Saos-2细胞的侵袭力明显增加（P<0.05），MMP-2分子的表达水平显著增加，与对照组相比，差异具有统计学意义（P<0.05）。结论 B7-H3基因可能通过调控MMP-2分子进而参与调节骨肉瘤的侵袭转移
%K 共刺激分子
%K B7-H3
%K 骨肉瘤
%K 侵袭
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%U http://www.zlfzyj.com/CN/abstract/abstract8530.shtml