siRNA干扰MTH1基因对肝癌HepG2细胞凋亡和迁移的影响
DOI: 10.3971/j.issn.1000-8578.2016.12.008
Keywords: 肝细胞癌,M T H 1,siRNA,凋亡,迁移,miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17,Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Effect of Down-regulation of HOXB7 Expression on Proliferation, Migration and Invasion Abilities of Hepatoma Cells,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Short-term Efficacy and Safety of HepaSphere Drug-loaded Microsphere Embolization on Primary Hepatocellular Carcinoma,Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism
Abstract:
摘要 目的 应用siRNA干扰技术抑制人肝细胞癌系HepG2细胞MTH1基因表达，并研究MTH1基因干扰后对HepG2细胞凋亡和迁移能力的影响。方法 设计并化学合成3对特异性MTH1-siRNA，采用阳离子脂质体法瞬间转染肝癌HepG2细胞，离体培养肝癌HepG2细胞，并设正常对照组、阴性对照组及MTH1-siRNA1、MTH1-siRNA2、MTH1-siRNA3转染组。采用蛋白印迹（Western blot）法检测转染MTH1-siRNA后HepG2细胞MTH1蛋白水平表达的变化，并筛选干扰效果最佳序列。运用流式细胞技术和划痕实验检测转染siRNA-MTH1后HepG2细胞凋亡、体外迁移能力的变化。结果 MTH1-siRNA3转染组干扰效果与正常组和阴性对照组相比效果具有统计学意义（P=0.002, P=0.005）。MTH1-siRNA3转染组细胞凋亡率高于正常组及阴性对照组，差异有统计学意义（P=0.012, P=0.013）；MTH1-siRNA3转染组细胞的划痕24、36 h愈合率均低于正常组和阴性对照组，差异有统计学意义（P=0.001, P=0.000）。结论 MTH1在肝癌细胞的凋亡及迁移中起重要作用，干扰MTH1的表达能促进肝癌细胞凋亡并降低肝癌细胞的迁移能力
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