%0 Journal Article
%T siRNA干扰MTH1基因对肝癌HepG2细胞凋亡和迁移的影响
%A 肖春辉
%A 谢春英
%A 赵丹
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.12.008
%X 摘要 目的 应用siRNA干扰技术抑制人肝细胞癌系HepG2细胞MTH1基因表达，并研究MTH1基因干扰后对HepG2细胞凋亡和迁移能力的影响。方法 设计并化学合成3对特异性MTH1-siRNA，采用阳离子脂质体法瞬间转染肝癌HepG2细胞，离体培养肝癌HepG2细胞，并设正常对照组、阴性对照组及MTH1-siRNA1、MTH1-siRNA2、MTH1-siRNA3转染组。采用蛋白印迹（Western blot）法检测转染MTH1-siRNA后HepG2细胞MTH1蛋白水平表达的变化，并筛选干扰效果最佳序列。运用流式细胞技术和划痕实验检测转染siRNA-MTH1后HepG2细胞凋亡、体外迁移能力的变化。结果 MTH1-siRNA3转染组干扰效果与正常组和阴性对照组相比效果具有统计学意义（P=0.002, P=0.005）。MTH1-siRNA3转染组细胞凋亡率高于正常组及阴性对照组，差异有统计学意义（P=0.012, P=0.013）；MTH1-siRNA3转染组细胞的划痕24、36 h愈合率均低于正常组和阴性对照组，差异有统计学意义（P=0.001, P=0.000）。结论 MTH1在肝癌细胞的凋亡及迁移中起重要作用，干扰MTH1的表达能促进肝癌细胞凋亡并降低肝癌细胞的迁移能力
%K 肝细胞癌
%K M T H 1
%K siRNA
%K 凋亡
%K 迁移
%K miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17
%K Effects of Sanguinarine on Invasion
%K Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells
%K Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells
%K Effect of Down-regulation of HOXB7 Expression on Proliferation
%K Migration and Invasion Abilities of Hepatoma Cells
%K Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer
%K Effects of miR-144 on Proliferation
%K Migration
%K Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells
%K Effect of ANLN Gene Silencing on Invasion
%K Migration
%K Proliferation and Apoptosis of Gastric Cancer Cells
%K Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro
%K Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells
%K Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3
%K Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line
%K Effects of Pachymaran on Proliferation
%K Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism
%K Effect of FBXL20 on Proliferation
%K Migration and Invasion of Gastric Cancer Cells
%K Short-term Efficacy and Safety of HepaSphere Drug-loaded Microsphere Embolization on Primary Hepatocellular Carcinoma
%K Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism
%U http://www.zlfzyj.com/CN/abstract/abstract8890.shtml