顺铂联合补中益气汤对A549/DDP荷瘤裸鼠移植瘤survivin蛋白表达的影响
DOI: 10.3971/j.issn.1000-8578.2016.11.004
Keywords: A Report of Two Cases and Literature Review,Relationship of Tumor Markers and Hilar Lymph Nodes with Bone Metastasis of Whole Body Bone Imaging in Lung Adenocarcinoma,Influence of CD151 Inhibition by RNAi on Lung Metastasis in Nude Mice Given Tail Veins Injection of Xuanwei Lung Adenocarcinoma XWLC-05 Cell Lines,HLA-G Expression in Lung Adenocarcinoma Tissues and Its Relationship with Prognosis,Antitumor Effect of Dihydroartemisinin on Hypopharyngeal Cancer in Nude Mice with Transplanted Tumor,Inhibition of Antimicrobial Peptide 17BIPHE2 on Lung Adenocarcinoma A549 Cells and Related Mechanism,Metabonomics Investigation of Lung Adenocarcinoma in Male Patients Based on Liquid Chromatography-mass Spectrometry,Napsin A, TTF1 and SPA Protein Expressions and Their Correlation with EGFR Gene Mutations in Primary Lung Adenocarcinoma,Expressions of Melanoma Antigen-As in Lung Adenocarcinoma Tissues and Related Clinical Significance,Correlation of Tumor Markers with Clinical Efficacy of Advanced Lung Adenocarcinoma Patients with EGFR Mutation,Expressions of SFRP1 Gene Methylation and SFRP1 Protein in Lung Adenocarcinoma and Related Clinical Significance,Expression of PTEN and EMMPRIN in Lung Adenocarcinoma Tissues and Their Correlation with EGFR Mutation
Abstract:
摘要 目的 探讨顺铂联合补中益气汤对A549/DDP荷瘤裸鼠移植瘤survivin蛋白表达的影响。方法 30只BALB/c-nu裸小鼠随机分为5组，A：荷瘤对照组、B：顺铂组、C：顺铂联合低剂量补中益气汤组、D：顺铂联合中剂量补中益气汤组、E：顺铂联合高剂量补中益气汤组。25 d后处死全部裸小鼠。测量移植瘤体积、重量并计算药物抑瘤率；Real-time PCR法检测移植瘤组织survivin mRNA的表达；Western blot法检测移植瘤组织survivin蛋白的表达。结果 顺铂联合低、中、高剂量组均较荷瘤对照组、顺铂组移植瘤体积显著缩小，重量显著减轻，差异有统计学意义；顺铂抑瘤率随着补中益气汤的剂量增加而增大；顺铂组、顺铂联合低剂量组较荷瘤对照组移植瘤组织中survivin的基因和蛋白表达上调，而顺铂联合中、高剂量组较荷瘤对照组survivin的基因和蛋白表达下调，差异均有统计学意义；顺铂联合低、中、高剂量补中益气汤组较顺铂组移植瘤组织中survivin的基因和蛋白表达均下调，差异均有统计学意义。结论 顺铂联合补中益气汤可通过下调survivin的表达改善A549/DDP荷瘤裸鼠移植瘤对顺铂的耐药性
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