%0 Journal Article
%T 顺铂联合补中益气汤对A549/DDP荷瘤裸鼠移植瘤survivin蛋白表达的影响
%A 井欢
%A 刘春英
%A 刘赞
%A 王哲
%A 王莹
%A 陈奇
%A 高原
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.11.004
%X 摘要 目的 探讨顺铂联合补中益气汤对A549/DDP荷瘤裸鼠移植瘤survivin蛋白表达的影响。方法 30只BALB/c-nu裸小鼠随机分为5组，A：荷瘤对照组、B：顺铂组、C：顺铂联合低剂量补中益气汤组、D：顺铂联合中剂量补中益气汤组、E：顺铂联合高剂量补中益气汤组。25 d后处死全部裸小鼠。测量移植瘤体积、重量并计算药物抑瘤率；Real-time PCR法检测移植瘤组织survivin mRNA的表达；Western blot法检测移植瘤组织survivin蛋白的表达。结果 顺铂联合低、中、高剂量组均较荷瘤对照组、顺铂组移植瘤体积显著缩小，重量显著减轻，差异有统计学意义；顺铂抑瘤率随着补中益气汤的剂量增加而增大；顺铂组、顺铂联合低剂量组较荷瘤对照组移植瘤组织中survivin的基因和蛋白表达上调，而顺铂联合中、高剂量组较荷瘤对照组survivin的基因和蛋白表达下调，差异均有统计学意义；顺铂联合低、中、高剂量补中益气汤组较顺铂组移植瘤组织中survivin的基因和蛋白表达均下调，差异均有统计学意义。结论 顺铂联合补中益气汤可通过下调survivin的表达改善A549/DDP荷瘤裸鼠移植瘤对顺铂的耐药性
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%U http://www.zlfzyj.com/CN/abstract/abstract8866.shtml