利托那韦对卡波氏肉瘤相关疱疹病毒感染细胞LYN表达的影响
DOI: 10.3971/j.issn.1000-8578.2017.03.003
Keywords: 卡波氏肉瘤相关疱疹病毒,利托那韦,LYN,脐静脉内皮细胞,PI3K/AKT,Effect of miR-7 Overexpression on Biological Behavior of Colon Carcinoma HCT-116 Cells,Research Progress on PI3K/AKT/mTOR Signaling Pathway in Burkitt Lymphoma,NOTCH Signaling Pathway Regulates Glioma Stem-like Cell Proliferation and Selfrenewal Abilities via PI3K/AKT Activity,L-Arginine Inhibites Proliferation of Human Lung Adenocarcinoma A549 Cells and Expression of PI3K/Akt Signaling Pathway,Effects on Cell Proliferation, Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway,Virulence Gene Mutation in Seven Kindreds of Lynch Syndrome in Yunnan Province,17-Allylamino-17-demethoxygeldanamycin Enhances Apoptosis Induced by Oxaliplatin via Suppression of Erk Signaling Pathway in RKO Colon Cancer Cell Line,Bez235,Inhibitor of Phosphoinositol-3-kinase/ mTOR,Inhibits SGC-7901 Cell Proliferation,Effects of Melatonin on Endothelial Cell Proliferation Induced by Lung Cancer Cell,Experiment of Vinorelbine or Combined with Hyperthermia on Inhibiting Angiogenesis,Effect ofRosiglitazone onExpression ofVEGF in LiverCancerCellLine SMMC-7721,Inhibitory Effect of 20(s)-Ginsenoside Rg3 on Proliferation and Migration of Vascular Endothelial Cells,LPA Inhibits Apoptosis Induced by Cisplatin in Ovarian Carcinoma Cells via PI3K/Akt Signaling Pathway,Regulationary of PTEN/PI3K/Akt Pathway on Apoptosis of K562 Cells
Abstract:
摘要 目的 探讨蛋白酶抑制剂利托那韦对卡波氏肉瘤相关疱疹病毒（Kaposi’s sarcoma associated herpesvirus, KSHV）感染细胞 LYN表达的影响。方法 原代培养人脐静脉内皮细胞HUVECs，从BCBL-1中提取KSHV病毒感染HUVECs，PCR扩增KS330233证实感染成功。CCK8法测定半数抑制浓度，CCK8法检测细胞增殖能力，Western blot检测LYN表达及p-PI3K和p-AKT水平。结果 RTV作用于细胞的IC50为25 μmol/L。RTV与LYN抑制剂PP2能抑制KSHV感染的HUVECs的增殖和LYN、p-PI3K和p-AKT的表达（P<0.05）。 结论 RTV可通过抑制LYN及其下游的PI3K/AKT信号通路抑制KSHV感染细胞HUVECs的增殖
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