%0 Journal Article
%T 利托那韦对卡波氏肉瘤相关疱疹病毒感染细胞LYN表达的影响
%A 姬玉
%A 张芳
%A 徐益明
%A 李冬妹
%A 李锋
%A 杨磊
%A 谭晓华
%A 郭峰
%A 陈颖
%J 肿瘤防治研究
%D 2017
%R 10.3971/j.issn.1000-8578.2017.03.003
%X 摘要 目的 探讨蛋白酶抑制剂利托那韦对卡波氏肉瘤相关疱疹病毒（Kaposi’s sarcoma associated herpesvirus, KSHV）感染细胞 LYN表达的影响。方法 原代培养人脐静脉内皮细胞HUVECs，从BCBL-1中提取KSHV病毒感染HUVECs，PCR扩增KS330233证实感染成功。CCK8法测定半数抑制浓度，CCK8法检测细胞增殖能力，Western blot检测LYN表达及p-PI3K和p-AKT水平。结果 RTV作用于细胞的IC50为25 μmol/L。RTV与LYN抑制剂PP2能抑制KSHV感染的HUVECs的增殖和LYN、p-PI3K和p-AKT的表达（P<0.05）。 结论 RTV可通过抑制LYN及其下游的PI3K/AKT信号通路抑制KSHV感染细胞HUVECs的增殖
%K 卡波氏肉瘤相关疱疹病毒
%K 利托那韦
%K LYN
%K 脐静脉内皮细胞
%K PI3K/AKT
%K Effect of miR-7 Overexpression on Biological Behavior of Colon Carcinoma HCT-116 Cells
%K Research Progress on PI3K/AKT/mTOR Signaling Pathway in Burkitt Lymphoma
%K NOTCH Signaling Pathway Regulates Glioma Stem-like Cell Proliferation and Selfrenewal Abilities via PI3K/AKT Activity
%K L-Arginine Inhibites Proliferation of Human Lung Adenocarcinoma A549 Cells and Expression of PI3K/Akt Signaling Pathway
%K Effects on Cell Proliferation
%K Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway
%K Virulence Gene Mutation in Seven Kindreds of Lynch Syndrome in Yunnan Province
%K 17-Allylamino-17-demethoxygeldanamycin Enhances Apoptosis Induced by Oxaliplatin via Suppression of Erk Signaling Pathway in RKO Colon Cancer Cell Line
%K Bez235
%K Inhibitor of Phosphoinositol-3-kinase/ mTOR
%K Inhibits SGC-7901 Cell Proliferation
%K Effects of Melatonin on Endothelial Cell Proliferation Induced by Lung Cancer Cell
%K Experiment of Vinorelbine or Combined with Hyperthermia on Inhibiting Angiogenesis
%K Effect ofRosiglitazone onExpression ofVEGF in LiverCancerCellLine SMMC-7721
%K Inhibitory Effect of 20(s)-Ginsenoside Rg3 on Proliferation and Migration of Vascular Endothelial Cells
%K LPA Inhibits Apoptosis Induced by Cisplatin in Ovarian Carcinoma Cells via PI3K/Akt Signaling Pathway
%K Regulationary of PTEN/PI3K/Akt Pathway on Apoptosis of K562 Cells
%U http://www.zlfzyj.com/CN/abstract/abstract8939.shtml