金丝桃苷诱导人胃癌细胞MKN-45凋亡及其机制
DOI: 10.3971/j.issn.1000-8578.2017.17.0461
Keywords: 金丝桃苷,MKN-45细胞,增殖,凋亡,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Autophagy Related Protein 5 Regulates Colon Cancer Cell Senescence Through p53/p21 Pathway,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism,Downregulation of Skp2 Expression Inhibits Malignant Phenotype of Osteosarcoma U2OS Cells,Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition,Effects of NDUFA4 Overexpression on Growth of Human Colon Cancer Cells in vitro
Abstract:
摘要 目的 探讨金丝桃苷（hyperoside, Hyp）对人胃癌MKN-45细胞增殖及凋亡的影响及其机制。方法 体外培养MKN-45细胞，对照组不加药物，实验组分别加入不同浓度Hyp（12.5、25、50、100、200 μg/ml）作用24、48 h，MTT法检测Hyp对细胞增殖的影响并筛选适宜药物浓度；流式细胞术检测细胞周期变化及凋亡率；Western blot法分析NF-κB P65、caspase-3、Bax、Bcl-2蛋白的表达情况。结果 Hyp可明显抑制MKN-45细胞的增殖，阻滞细胞于G0/G1期并促进其凋亡，Western blot结果显示，随着药物浓度的增高，Hyp可使NF-κB P65、Bcl-2蛋白表达降低，casepase-3、Bax蛋白表达增高。结论 金丝桃苷可抑制MKN-45细胞增殖并诱导凋亡，其机制可能与该药物阻断细胞周期，抑制NF-κB通路，下调NF-κB P65 、Bcl-2蛋白，上调casepase-3、Bax蛋白有关
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