%0 Journal Article
%T 金丝桃苷诱导人胃癌细胞MKN-45凋亡及其机制
%A 刘海雯
%A 郭莲怡
%J 肿瘤防治研究
%D 2017
%R 10.3971/j.issn.1000-8578.2017.17.0461
%X 摘要 目的 探讨金丝桃苷（hyperoside, Hyp）对人胃癌MKN-45细胞增殖及凋亡的影响及其机制。方法 体外培养MKN-45细胞，对照组不加药物，实验组分别加入不同浓度Hyp（12.5、25、50、100、200 μg/ml）作用24、48 h，MTT法检测Hyp对细胞增殖的影响并筛选适宜药物浓度；流式细胞术检测细胞周期变化及凋亡率；Western blot法分析NF-κB P65、caspase-3、Bax、Bcl-2蛋白的表达情况。结果 Hyp可明显抑制MKN-45细胞的增殖，阻滞细胞于G0/G1期并促进其凋亡，Western blot结果显示，随着药物浓度的增高，Hyp可使NF-κB P65、Bcl-2蛋白表达降低，casepase-3、Bax蛋白表达增高。结论 金丝桃苷可抑制MKN-45细胞增殖并诱导凋亡，其机制可能与该药物阻断细胞周期，抑制NF-κB通路，下调NF-κB P65 、Bcl-2蛋白，上调casepase-3、Bax蛋白有关
%K 金丝桃苷
%K MKN-45细胞
%K 增殖
%K 凋亡
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%U http://www.zlfzyj.com/CN/abstract/abstract9074.shtml